R. M. F. Guinet

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A non-competitive avidin-biotin immuno-enzymatic assay (NABA) for lysozyme is described. The assay was found to be more sensitive than a competitive assay with biotinylated lysozyme. The lower detection limit of NABA was 0.1 ng lysozyme/ml compared to 1 ng/ml for the competitive assay. The intra-assay and inter-assay coefficients of variation for NABA were(More)
A rapid method has been standardized for extraction and SDS-PAGE analyses of mycobacterial cellular protein. Complex patterns were reproducibly obtained of the 31 reference strains or clinical isolates studied, belonging to 11 species. These patterns were species specific; thus this method could be an easy tool for mycobacterial strain characterization.
Emerging yeast pathogens are favoured by increasing numbers of immunocompromised patients and by certain current medical practices. These yeasts differ in their antifungal drug susceptibilities, and rapid species identification is imperative. A large variety of methods have been developed with the aim of facilitating rapid, accurate yeast identification.(More)
A simple electrophoretic method for yeast identification was evaluated. Whole cells were extracted by SDS and the protein profiles obtained in SDS-PAGE after Coomassie blue staining were compared for 52 strains from 9 species of yeast or yeast-like fungi commonly isolated from man (Candida albicans, C. glabrata, C. guilliermondii, C. krusei, C.(More)
Over the past several decades there has been a significant increase in the number of fungal diseases. Although infections can occur in normal hosts, most of them are seen in patients who are immunosuppressed or immunocompromised such as those with AIDS or who have received transplants, corticoids or anticancer drugs [1]. Other predisposing factors(More)
SETTING In 1990, a 6-month short-course regimen (2 SHRZ/4 RH) was introduced for the treatment of tuberculosis in Morocco. OBJECTIVE To assess the efficacy of the national tuberculosis control programme, a prospective study of primary drug resistance was conducted from April 1992 to July 1994 in Casablanca. DESIGN A total of 402 strains isolated from(More)
Two different microtiter plate ELISA tests were devised for the detection of Escherichia coli thermolabile toxin (LTh) either free or extracted from isolated colonies. Both tests used as detection systems purified anti-LTh rabbit immunoglobulins conjugated to biotin, streptavidin peroxidase and TMB. The tests differed by their capture phase which was the(More)
The immunological detection of soluble pneumococcal polysaccharide antigens in pathological products is of importance in the direct diagnosis of meningitis or pulmonary infections. We have developed a double antibody sandwich ELISA method using a biotin-avidin system using antibodies constituted with a mixture of IgGs from pooled and/or monospecific(More)
Experiments with a semi-automated susceptibility test system are described. The system was based on a laser nephelometer which was used to estimate extinction in broth cultures, and ready-to-use cuvettes containing antibiotics. Each antibiotic was tested at two concentrations, the cuvettes were manually inoculated and the results were obtained after 3-5 h(More)
The performance of a modified Mycotube test for the identification of yeasts was evaluated using standard biochemical tests as reference. One hundred and eighty strains belonging to 12 medically important species (15 strains each) were tested. The overall rate of identification was 72%, and rose to 87.8% when macroscopic and microscopic morphologic features(More)