R Langlois van den Bergh

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We prospectively studied the reconstitution of lymphocyte subpopulations in a group of 22 children, who survived disease-free at least 6 months after allogeneic BMT for a haematological malignancy. Absolute counts of total lymphocytes, B lymphocytes, T lymphocytes, and CD4+ helper T lymphocytes reached the 5th percentile (p5) of age-matched reference values(More)
A direct immunofluorescence staining of either T or B lymphocyte membrane markers was combined with a quinacrine staining for the Y-chromatin in the interphase nuclei of these cells. In this way it was possible to identify the origin of both lymphocytic populations in the peripheral blood after a successful bone marrow transplantation from a donor of the(More)
A powerful approach to documenting engraftment after allogeneic BMT is the quantification of the degree of chimaerism in distinct haematopoietic cell lineages. This cannot be achieved by the recently developed, quantitative, modifications of PCR amplification of highly polymorphic DNA markers, unless this technique is applied to separated cell populations.(More)
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