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This paper analyzes whether the decreasing progressivity of the US Federal Tax system appear to affect taxpayer satisfaction. I use data from the publicly available General Social Survey and combine them with average tax rates from Piketty and Saez (2007). In this study, two distinct questions are addressed: (1) Is a taxpayer's belief that his or her own(More)
This paper describes a method to reconstitute GPCRs into an array of giant liposomes in a buffer solution. We first achieved the formation of giant liposomes in a buffer solution by adding fructose to a lipid film; normally such giant liposomes are formed by using only pure waters (MilliQ). Moreover, we successfully reconstituted adrenergic receptors (AR)(More)
This paper describes the electrical detection of single-stranded DNA (ssDNA) at the single molecule level using an engineered alamethicin (Alm) channel embedded in bilayer lipid membranes (BLMs). Nanopore sequencing has the potential to become a direct, fast and inexpensive DNA sequencing methodology (Fig. 1). We propose here the chemically covalent dimmers(More)
This paper describes a methodology that realizes an array of heterologous giant liposomes with gentle hydration. The system consists of an array of micropores on a thin polymer film, which is overlaid on a pair of comb ITO-electrodes. An electrospray deposition (ESD) method allows selective lipid patterning of green and red fluorescent-labeled lipids at the(More)
This paper investigates the properties of DNA translocations signals in a stochastic framework. The considered signals are relative to the translocation of single strand DNA through natural nanopores, and are obtained using a planar patch clamp method. The stochastic signal analysis is carried out considering the statistical distribution of DNA(More)
We have developed a fluorescence-based microfluidic system for high-throughput analyses of the function of ATPbinding cassette (ABC) transporters. Our device consists of polydimethylsiloxane (PDMS) and a glass that tethers vesicles on its surface. Although PDMS is a great material for fabrication of microfluidic channels, it absorbs fluorescent molecules(More)
This paper describes an instant formation method of uniform-sized proteoliposomes for membrane protein assays using fluorescence microscopy. The applied electrospray technique was the same as our previous works [1], although here we directly sprayed proteovesicles (<1 μm size) dispersed in “an aqueous buffer” and micropatterned them on a substrate;(More)
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