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Evidence for the occurrence of indole 3-acetic acid in Caulerpa paspaloides extracts was obtained by bioassay, by high-performance liquid chromatography with an electrochemical detector, and by capillary gas chromatography combined with mass spectrometry. The amount of indole 3-acetic acid present was estimated to be about 1 milligram per kilogram fresh(More)
Zimmerer, Robert P. (The Pennsylvania State University, University Park), Robert H. Hamilton, and Christine Pootjes. Isolation and morphology of temperate Agrobacterium tumefaciens bacteriophage. J. Bacteriol. 92:746-750. 1966.-Lysogeny was detected in 14 strains of Agrobacterium tumefaciens among 130 bacterial strains tested with strain B-6 used as the(More)
An auxin-requiring soybean root callus metabolized [1-(14)C]-2,4-dichlorophenoxyacetic acid (2,4-D) to diethyl ether-soluble amino acid conjugates and water-soluble metabolites. The uptake in tissue varied with incubation time, concentration, and amount of tissue. Uptake was essentially complete (80%) after a 24-hour incubation and the percentage of free(More)
Soybean (Glycine max L.) cotyledon callus grown on radioactive 2,4-dichlorophenoxyacetic acid (2,4-D-1-(14)C) as an auxin produced 2,4-D metabolites, which qualitatively and quantitatively changed with time. Water soluble fractions from the tissue exhibited a steady increase in radioactivity during the course of 24 days. Following beta-glucosidase(More)
The biological activity of 20 l-alpha-amino acid conjugates of indole-3-acetic acid (IAA) to stimulate cell elongation of Avena sativa coleoptile sections and to stimulate growth of soybean cotyledon tissue cultures has been examined at concentrations of 10(-4) to 10(-7)m. In the Avena coleoptile test, most of the amino acid conjugates stimulated(More)
Only quantitative differences were observed in the metabolism of [1-(14)C]2,4-dichlorophenoxyacetic acid (2,4-D) by soybean cotyledon callus as influenced by variety (Acme and Amsoy), source of tissue (cotyledon, leaf and root) and age. All tissues metabolized 2,4-D to water-soluble (glycosides) and ether-soluble (primarily amino acid conjugates)(More)