R. Gary Kirk

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Cryosections of human red blood cells infected by Plasmodium falciparum were analyzed by energy dispersive x-ray microanalysis to determine the elemental composition of the parasites and their red cell hosts separately. The effects of two antimalarial drugs, qinghaosu and chloroquine, on potassium, sodium, and phosphorus concentrations were studied.(More)
To investigate the mechanism of salt secretion in the avian salt gland, we used quantitative electron probe microanalysis to measure the intracellular elemental concentrations in dry cryosections of unspecialized and partially specialized secretory epithelial cells from fresh water- and salt water-adapted ducklings, respectively. In conjunction with this,(More)
UNLABELLED This study examined 1) whether potassium-induced depression of phosphate excretion is a parathyroid hormone-dependent phenomenon, and 2) whether such stimulation of tubular phosphate reabsorption capacity involves increased phosphate reabsorption in the distal tubule. Potassium was infused into intact rats (25 mumol X min-1 X kg-1) during(More)
Freeze-fracture electron-microscopy has been used to study membrane ultrastructure in (1) red cells from five species of mammals which have naturally occurring differences in cation transport and (2) red cells which have been treated with various drugs known to affect transport. A reproducible method for estimation of the intramembrane particle density is(More)
Potassium influxes in red cells from eight species have been found to follow exponential relationship with membrane phosphatidylcholine and sphingomyelin content. This relationship with membrane phosphatidylcholine and sphingomyelin content. This relationship with membrane phospholipid patterns was found to exist with both ouabain sensitive and insensitive(More)
The concentrations of potassium, sodium, and iron in human and sheep red blood cells were measured with an electron probe. Cells were prepared for analysis by spraying them on pyrolytic graphite supports. The results obtained with this spray technique agreed well with values measured on similar cells that were prepared for analysis by freezing, sectioning,(More)
Red blood cells of certain species of animals, such as dogs and cats, contain low potassium and high sodium, whereas the erythropoietic stem cells giving rise to these cells are of high potassium type. This paper examines the sequence of membrane transport changes during erythropoiesis by analyzing the K, Na and Fe in single bone marrow cells, reticulocytes(More)
Treatment with the iodine-containing antiarrhythmic drug, amiodarone, can cause pulmonary toxicity. Alveolar macrophages are particularly susceptible to formation of lipidrich lamellar bodies in amiodarone-treated animals. Amiodarone and several of its metabolites accumulate in the cell. Previously, we have reported that the technique of X-ray microanalysis(More)
When administered to humans and animals, the iodine-containing drug amiodarone can cause pulmonary toxicity. As part of the pulmonary response to amiodarone, the drug and its principal metabolite, desethylamiodarone, accumulate in alveolar macrophages. Little is known about the susceptibility of lungs with preexisting damage to amiodarone administration. A(More)