A method is described for the detection of DNA hybrids formed on a solid support, based upon the pairing of oligonucleotide chemistry and the technologies of electronic microdevice design. Surface matrices have been created in which oligonucleotide probes are covalently linked to a thin SiO2 film. 32P labeled target nucleic acid is then hybridized to this… (More)
This report describes the second release of the Rutherford Appleton Laboratory Mesh Partitioning Program ralpar. Ralpar is a software tool to split up unstructured meshes into subdomains for parallel processing on MIMD type architectures. A wide range of basic decomposition methods have been implemented within this tool. This document briefly describes… (More)
New technologies for multiple chemical synthesis of oligonucleotides and stepwise hybridization on a solid-phase support enable the rapid and cost-effective preparation of long duplex DNA regions. Will these new technologies usher in a new era in protein engineering?
This report provides a survey of some of the software tools currently available to assist in the development of Fortran programs using the new 1990 and 1995 standards. A few tools also address the development of High Performance Fortran (HPF). This report is particularly concerned with tools which will enhance the quality of software developed. We have not… (More)
We present results from a three-dimensional device simulator, using adaptive meshing and solving the drift-diffusion equations. The adaption algorithm and the criteria used for adap-tion are discussed. Three devices of industrial interest are presented: a bipolar transistor, a 1.25/zm n-MOS device and a CCD, illustrating the range of devices which may be… (More)
One variant of a complex satellite DNA of the Bermuda land crab is significantly longer than the average repeat unit of the satellite. The extra DNA in the variant is accounted for by a fivefold tandem amplification of a 0.142-kilobase sequence. The amplified sequence is bounded by a tetranucleotide inverted repeat; the upstream arm of the inverted repeat… (More)