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Micro-complement fixation studies of eastern and western populations of the North American tree frog Hyla chrysoscelis reveal they have been genetically isolated for about 4 million years. Immunological comparisons of populations of the cryptic tetraploid Hyla versicolor indicate a recent origin, from hybridization between eastern and western H.(More)
The quantitative immunological technique of micro-complement fixation (MC'F) has been routinely used during the past decade to assess evolutionary relationships among living vertebrate species. The large data base that has been generated, along with the excellent correlations between immunologically measured genetic distances and paleontologically derived(More)
We have previously put forward a hypothesis that explains how and why micro-complement fixation (MC'F) works (Maxson and Maxson 1986). This is called the assortment-exclusion hypothesis and can explain the high sensitivity of the MC'F reaction, justify the logarithmic basis for immunological distance (ID), and confirm the presumed phylogenetic nature of the(More)
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