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The anchorage-dependent and anchorage-independent growth of the human mammary carcinoma cell line MDA-MB-231 is inhibited by vitamin A (retinol). Clones resistant to growth inhibition by retinol were isolated from this cell line in soft agar without the use of mutagens. This paper describes the isolation and characterization of the resistant lines. The(More)
Methods Neutralizing antibodies against sAd24 were determined using a panel of 106 rhesus macaque sera and 128 human sera from Rwanda and South Africa using a luciferasebased adenovirus neutralization assay. The immunogenicity of a single dose of 10E7, 10E8 or 10E9 virus particles of sAd24-SIV Gag based vector was determined in C57BL/6 mice.(More)
Background The immunogenicity of adenovirus serotype 5 (Ad5) vectors is suppressed by pre-existing neutralizing antibodies (NAbs) that are directed primarily against the hexon hypervariable regions (HVRs). We previously reported that replacing all 7 HVRs of the Ad5 hexon protein with those from the rare serotype Ad48 resulted in a chimeric Ad5HVR48(1–7)(More)
In humans, the plasma enzyme, butyrylcholinesterase (E.C., metabolizes cocaine to the water-soluble, pharmacologically inactive compounds, ecgonine methylester and benzoic acid. Homogeneous enzyme was purified from human plasma and used to determine the enzyme kinetic parameters of Km and Vmax with cocaine as the substrate. The KM (11.9 microM)(More)
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