R A R Bywater

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Spontaneous contractions were recorded from the circular muscle layer at three sites along the isolated mouse colon. The interval between contractions was approximately 4.5 min. The mean duration of the contractions ranged from 26 sec in the distal colon to 45 sec in the proximal colon. Contractions migrating more than half the length of the colon were(More)
BACKGROUND/AIMS Little is known about the mechanisms controlling colonic migrating electrical activity. This study investigates the neural processes involved in the generation of migrating myoelectric complexes in the isolated mouse colon. METHODS Intracellular electrophysiological recordings were obtained from the circular muscle layer of the mouse colon(More)
This study has used mechanical, together with pressure/volume recordings or electrophysiological recordings, to investigate the spontaneous activity in isolated preparations of mouse colon. In the former preparations, when not distended with fluid, spontaneous colonic migrating motor complexes (CMMCs) were observed using isotonic transducers. When the(More)
1. Electrotonic potentials were recorded from the superficial smooth muscle cells of the guinea-pig vas deferens using the method of Abe & Tomita (1968), in response to low-amplitude, long-duration (greater than or equal to 2 sec) pulses. 2. Averaging techniques were used to increase the signal/noise ratio, and the intracellularly recorded electrotonic(More)
Smooth muscle cells enzymatically dispersed from the circular muscle layer of the guinea pig colon were examined for the expression of voltage-gated ionic currents using the whole cell patch-clamp technique. Three outward currents and one inward current were identified and characterized. The inward current, at physiological potentials, was generated by a(More)
Intracellular recordings have been made in vitro from the myenteric neurons of the distal colon of normal littermates of the piebald-lethal mouse. Out of a total of 90 neurons, 82 were classified as S/type 1 cells and 8 as AH/type 2 cells. Seventy-eight out of 82 S cells showed spontaneous fast excitatory postsynaptic potentials (EPSPs) sensitive to(More)
Intracellular electrophysiological techniques were used to record the spontaneous myoelectric activity in the circular muscle layer of an in vitro preparation of whole mouse colon. In 34 out of 58 preparations, spontaneous depolarisations (myoelectric complexes, MCs) were recorded cyclically, about every 4 min. In these preparations, apamin (250 nM) and(More)
Intracellular microelectrodes were used to record electrically evoked inhibitory junction potentials (IJPs) and electrotonic potentials during spontaneous cyclical depolarisations (myoelectric complexes, MCs) in the circular muscle layer of mouse colon in vitro. In the presence of nifedipine (1-2 microM) and atropine (1 microM), MCs were recorded every 264(More)
In this investigation the characteristics of the response to colonic distension of afferent fibres projecting to the inferior mesenteric ganglion (IMG) of the guinea pig were studied in vitro. Intracellular membrane potential recordings were made from neurons in the IMG. Post-synaptic potentials arising from activity in afferent fibres were recorded in(More)
Spontaneous colonic migrating motor complexes (CMMCs) were recorded from circular muscle at three sites along the isolated mouse colon. The interval between CMMCs was decreased from approximately 3 min in control solution, by approximately 55% in a nitric oxide synthase (NOS) inhibitor, N-nitro-L-arginine (L-NNA; 100 micromol L-1). This was associated with(More)