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In order to facilitate high throughput genotyping of zebrafish, we have developed a novel technique that uses High Resolution Melting Analysis (HRMA) to distinguish wild-type, heterozygous mutants and homogyzous mutants. This one hour technique removes the need for restriction enzymes and agarose gels. The generated melting curve profiles are sensitive(More)
OBJECTIVE To report a case of postoperative necrosis after conservative management of torsion of a hyperstimulated ovary. DESIGN Case report and literature review. SETTING Tertiary care center. PATIENT Infertility patient undergoing IVF-ET. INTERVENTIONS Detorsion followed by unilateral salpingo-oophorectomy 2 days later. MAIN OUTCOME MEASURE(More)
A continuous-flow, temperature gradient microfluidic device was used to demonstrate spatial DNA melting analysis with the resolution and reproducibility necessary for clinical SNP scanning and genotyping of human genomic DNA. With a steady-state temperature gradient of 20-30 degrees C across a sample, melting curves were constructed from a single(More)
This paper introduces an innovative method for genotyping 1-2 days old zebrafish embryos, without sacrificing the life/health of the embryos. The method utilizes microfluidic technology to extract and collect a small amount of genetic material from the chorionic fluid or fin tissue of the embryo. Then, using conventional DNA extraction, PCR amplification,(More)
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