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In this study three major subtypes of nicotinic acetylcholine receptors were characterized pharmacologically using the calcium influx through the ion channel as a robust functional assay system. Human α3β4 receptors and α4β2 receptors were cloned and stably expressed in HEK293 cells. [125I]epibatidine saturation binding yielded a B max of 4420±840 fmol/mg(More)
A simple procedure for removing methacrylic acid from water-miscible methacrylates is described. One volume of monomer is diluted with 9 volumes of diethyl ether. Three consecutive extractions are carried out with 5% aqueous NaHCO3 at 4 C. Residual water is removed by shaking with anhydrous sodium sulfate. The ether is removed by flash evaporation on a(More)
In this study three major subtypes of nicotinic acetylcholine receptors were characterized pharmacologically using the calcium influx through the ion channel as a robust functional assay system. Human alpha3beta4 receptors and alpha4beta2 receptors were cloned and stably expressed in HEK293 cells. [(125)I]epibatidine saturation binding yielded a B(max) of(More)
Corticotropin-releasing factor (CRF) is a hypothalamic 41-amino acid peptide which stimulates corticotropin (ACTH) release from the anterior pituitary and is also involved in the body response to stress. CRF1 receptors represent a potential target for novel antidepressant/anxiolytic drugs. The aim of the present study was to search for a human cell line(More)
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