Qiu-lin Yang

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OBJECTIVE To detect Toxoplasma gondii DNA by loop-mediated isothermal amplification (LAMP). METHODS DNA was extracted by phenol-chloroform extraction from T. gondii tachyzoites. Four primers which recognized 6 distinct regions on the B1 gene of T. gondii were designed and used for LAMP assay. To evaluate the specificity of the method, Plasmodium vivax, P.(More)
This work describes the structural changes of bagasse hemicelluloses during the cooking process involving active oxygen (O(2) and H(2)O(2)) and solid alkali (MgO). The hemicelluloses obtained from the bagasse raw material, pulp, and yellow liquor were analyzed by high-performance anion-exchange chromatography (HPAEC), gel permeation chromatography (GPC),(More)
To develop a reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay for rapid and sensitive detection of Norwalk GII. 4 primers which recognized 6 distinct regions on the RNA-dependent RNA polymerase gene of Norwalk GII were designed and used for LAMP assay. Norwalk GII RNA was amplified under isothermal conditions (65 degrees C) for(More)
The structural characteristics of corn stalks hemicelluloses during the active oxygen cooking process as a pretreatment of biomass conversion were investigated in this work. The hemicelluloses obtained from the corn stalks, pulp, and yellow liquor were evaluated by high-performance anion-exchange chromatography (HPAEC), Fourier transform infrared(More)
The root barks of Sophora japonica L., a plant widely used in traditional Chinese medicine, were extracted with 70 % Me2CO. The antibacterial activity of the crude extracts and fractions from the subsequent purification was evaluated against two Gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus) and two Gram-negative bacteria (Klebsiella(More)
OBJECTIVE To acquire and analyze adult stage Schistosoma japonicum (Chinese strain) expressed sequence tags and new genes from an adult S. japonicum cDNA library, and to search new vaccine candidates and drug targets. METHODS A cDNA library was constructed from adult stage S. japonicum. Clones were selected randomly from the cDNA library and were(More)
This paper applies a global sliding mode variable structure control method based on neural network reaching law to Permanent Magnet Synchronous Motor. Parameters, ε and K, which were determined previously in the conventional reaching law, are regulated adaptively by two back-propogation neural networks (bpNN) respectively. Thus, a new global sliding(More)
A recombinant plasmid containing cathepsin B endopeptidase of Schistosoma japonicum (Sjcb2) was constructed, indentified by PCR, restrictive enzyme, digestion and DNA sequencing, and expressed into mammalian cells. Immunochemistry examination showed that the Sjcb2 gene can be expressed in the eukaryotic system, providing a basis for the development of(More)
Sj20.8 gene was amplified by PCR and inserted into eukaryotic expression plasmid pcDNA3.1 to construct recombinant plasmid pcDNA3.1/Sj20.8, which was then injected into the quadriceps femoris of the BALB/c mice. Results showed that the Sj20.8 antigen was low expressed in the local tissue of the mice, and was not able to significantly reduce eggs in the(More)
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