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Microbial production of R-3-hydroxybutyric acid by recombinant E. coli harboring genes of phbA, phbB, and tesB
TLDR
TesB, generally regarded as a medium chain length acyl CoA thioesterase, was found, for the first time, to play an important role for transforming short chain length 3-hydroxybutyrate-CoA to its free fatty acid, namely, 3HB.
Production of polyhydroxyalkanoates with high 3-hydroxydodecanoate monomer content by fadB and fadA knockout mutant of Pseudomonas putida KT2442.
TLDR
Thermal and mechanical properties assays indicated that this new type PHA with a high HDD fraction had higher crystallinity and tensile strength than P HA with a low HDD fraction did, demonstrating an improved application property.
Overexpression of NAD kinase in recombinant Escherichia coli harboring the phbCAB operon improves poly(3-hydroxybutyrate) production
TLDR
Results clearly showed that the overexpression of NAD kinase could be used to enhance the PHB synthesis, which is one of the most crucial factors influencing PHB production.
Production of poly-3-hydroxybutyrate by Bacillus sp. JMa5 cultivated in molasses media
TLDR
It seems that sporulation induced by reduced supply of nutrients is the reason that PHB content is generally low in the Bacillus sp.
Biosynthesis and characterization of poly(3-hydroxydodecanoate) by β-oxidation inhibited mutant of Pseudomonas entomophila L48.
TLDR
In this study, some of the genes encoding key enzymes in β-oxidation cycle of P. entomophila such as 3-hydroxyacyl-CoA dehydrogenase, 3-ketoacyl -CoA thiolase, and acetyl- coA acetyltransferase were deleted to study the relationship between β-Oxidation and PHA synthesis in P. entrants.
Microbial production and applications of chiral hydroxyalkanoates
TLDR
The physiological functions of (R)-3-hydroxybutyric acid (3HB) and (R-4-hydroxydecanoic acid are reviewed and the technologies developed to produce various HA and the applications of chiral HA are summarized.
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