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Cloning and characterization of a plastidic glycerol 3-phosphate dehydrogenase cDNA from Dunaliella salina.
It is suggested that the phosphoserine phosphatase domain functions as glycerol 3-phosphatase and that, consequently, NAD+ -dependent GPDH from D. salina can catalyze the step from dihydroxyacetone phosphate to Glycerol directly directly. Expand
Cloning and expression study of a putative high-affinity nitrate transporter gene from Dunaliella salina
A nitrate transporter gene, named DsNRT2.1 (GeneBank accession number AY621079), from Dunaliella salina has been cloned by screening a cDNA library, which was constructed with mRNAs from D. salina as a probe and showed sequence similarity to those known nitrate transporters of the NRT2 family. Expand
Purification and characterization of a hydrolysis‐resistant lipase from Aspergillus terreus
Lipase from Aspergillus terreus was purified to homogeneity using ammonium sulfate precipitation and chromatographies with Q‐Sepharose and Sephacryl S‐200 and revealed that the Ser was involved in catalysis. Expand
Transcriptomic profiles of Dunaliella salina in response to hypersaline stress
The response of Dunaliella salina to hypersaline stress is a systematic work, possibly involving enhancements of photosynthesis, carbon fixation, and heme biosynthetic process, acceleration of protein turnover, spliceosome, protein processing in endoplasmic reticulum, and endocytosis, as well as degradation of starch, synthesis of glycerol, membrane lipid desaturation. Expand
Correlations between the polymorphism of +869T/C in TGF-β1 and rheumatoid arthritis
The polymorphism of the gene first exon +869T/C in TGF-β1 significantly correlated with RA and CC genotype might be the susceptible gene of RA. Expand
Cloning and Characterization of Multiple RNA Splicing Variants of LDH-C Gene in Human and Rat
The results indicate that LDH-C was not silenced by transcriptional repression in non-mature germ cells, but significantly transcripted and alternatively spliced, which suggested that these variants are possibly not used for translation, but targets of nonsense-mediated mRNA decay. Expand
[Isolation and purification of alpha-glycerophosphate oxidase in a polyethylene glycol/(NH4 )2SO4 aqueous two-phase system].
Alpha-glycerophosphate oxidase (alpha-GPO) from Enterococcus casseli flavus was successfully isolated and purified by using polyethylene glycol (PEG)/(NH4)2SO4 aqueous two-phase system (ATPS). TheExpand
Inhibitor screening of lactate dehydrogenase C4 from black-lipped pika in the Western Sichuan Plateau
Virtual screening and in vitro enzyme assay using pika LDH-C4 as target protein revealed NSC61610, NSC215718, and NSC345647 with Ki of 7.8, 27, and 41 μM separately might be helpful for development of pika contraceptive drugs. Expand
Characterization and Inhibitor Screening of Plateau Zokor Lactate Dehydrogenase C4
Virtual and in vitro screening against zokor LDH-C4 revealed eight N-substituted oxamic acids with IC50s ranging from 198 to 2513 μM, higher than that of oxamic acid and (ethylamino)(oxo)acetic acid, and the increase in the length of substituting chain seems not to increase inhibition potency. Expand