Qing-li Cheng

Learn More
Age-associated renal changes may be an important cause of renal failure. We recently found that aged female B6 mice developed progressive glomerular lesions. This was associated with macrophage infiltration, a frequent finding in glomerulosclerosis. We used these mice as a model for studying the mechanisms of glomerular aging. We compared the gene(More)
Immunofluorescence of frozen tissue sections (IF-F) is a classic technique for renal immunopathologic examination. However, it has certain disadvantages, such as diffuse antigen distribution and few or even no glomeruli in the section. We developed a new technique of immunofluorescence staining using dual microwave retrieval in paraffin-embedded renal(More)
OBJECTIVES To investigate the incidence, pathogenetic factors, the prognosis and correlation factors of the hospitalized very elderly patients(=80) with acute kidney injury (AKI). METHODS The clinical data of the patients older than 80 admitted in PLA General Hospital from June 1, 2008 to December 31, 2009 were collected. The patients with AKI were(More)
The pathologic hallmarks of diabetic nephropathy are excess mesangial extracellular matrix (ECM) and mesangial cell proliferation. We previously showed that mesangial cell phenotypic changes play an important role in the pathogenesis of diabetic nephropathy. We concluded that phenotypic changes were present in bone marrow (BM)-derived mesangial cell(More)
The incidence of acute kidney injury in patients with diabetes is significantly higher than that of patients without diabetes, and may be associated with the poor stemness capacity of kidney stem cells (KSCs) and limited recovery of injured renal tubules. To investigate the effects of hyperglycemic stress on KSC stemness, KSCs were isolated from the rat(More)
OBJECTIVE Evidence has demonstrated that Ca(2+)/calmodulin-dependent protein kinase type IV (CaMKIV) contributes to altered cytokine production by promoting the production of inflammatory cytokines. This study aimed to explore the protective role and underlying mechanisms of CaMKIV inhibition in experimental nephrotic syndrome. METHODS BALB/c mice(More)
OBJECTIVE To investigate the effects of atorvastatin (AVT) on renal function and renal pathological changes in the aged rat and explore their possible mechanisms. METHODS Twenty-month-old, normal female Wistar rats were divided into three groups: group A (n=8) was fed high-dose AVT (10mg/kg/d); group B (n=8) was fed low-dose AVT (1mg/kg/d); and group C(More)
The glomeruli of postmenopausal C57BL6 mice, and age-matched males, show progressive hypertrophy and glomerulosclerosis. We asked whether this was a multistage process, was due to alterations in glomerular progenitors, and was reversible in female mice. Using cross bone marrow transplants (BMT) between young and old females, we found that BMT delivered a(More)
BACKGROUND Immunofluorescence of frozen tissue sections (IF-F) is a traditional technique used in renal biopsy. However, IF-F has certain disadvantages, such as a few or even no glomeruli in the section, and limited long-term preservation of the fluorescently labelled samples. METHODS We compared two-step immunohistochemistry (IHC) staining of(More)
  • Sheng Liu, Qing-Li Cheng, +4 authors Xiao-Yan Cai
  • International journal of clinical and…
  • 2015
This study aims to analyze the factors that affect the prognosis of continuous renal replacement therapy (CRRT) in elderly patients with acute kidney injury (AKI). Data obtained from 41 elderly patients with AKI who underwent CRRT in our department between January 2001 and December 2010 was retrospectively evaluated in this study. The enrolled patients were(More)