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In this study, we developed a real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) method to study cytochrome P450 (CYP) mRNA regulation by cytokines in mouse liver. The method combines standard RT-PCR with a fluorogenic probe in which the intensity of fluorescence is proportional to the amount of target template present. We show(More)
1. The aim was to employ real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) technology (TaqMan to examine the induction of some selected cytochrome P450 (CYP) forms in precision-cut rat liver slices. 2. Taqman primers and probe sets were developed for rat CYP1A1, CYP1A2, CYP2B1 and CYP4A1 forms. 3. Rat liver slices were cultured(More)
Using a spread of PG(3; p) and certain projective two-weight codes, we give a general construction of Hadamard diierence sets in groups H (Z p) 4 , where H is either the Klein 4-group or the cyclic group of order 4, and p is an odd prime. In the case p 3 (mod 4), we use an ovoidal bration of PG(3; p) to construct Hadamard diierence sets, this construction(More)
By modifying the constructions in [10] and [15], we construct a family of cyclic ((q 3k − 1)/(q − 1), q − 1, q 3k−1 , q 3k−2) relative difference sets, where q = 3 e. These relative difference sets are " liftings " of the difference sets constructed in [10] and [15]. In order to demonstrate that these relative difference sets are in general new, we compute(More)