Qing-Song Han

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AIM To determine alterations of subtypes of myocardial adrenoceptors in senescence. METHODS Heart membrane preparations were made from 3- and 25-month old Wistar rats. Alpha 1- and beta-adrenoceptors were measured by radioligand, 125I-BE2254 and 125I-pindolol, binding assays, respectively. RESULTS In the old rat heart, alpha 1- and beta-adrenoceptor(More)
The antagonistic effect of tetrahydroproberberine (THP) homologues on alpha 1-adrenoceptor was studied by combination of radioligand binding assays and measurements of vasoconstriction responses. The results showed that l-tetrahydropalmatine (l-THP), l-stepholidine (l-SPD), THPB-18 and tetrahydroberberine (THB) competitively inhibited the 125I-BE2254(More)
Grancalcin is a Ca(2+)-binding protein expressed at high level in neutrophils. It belongs to the PEF family, proteins containing five EF-hand motifs and which are known to associate with membranes in Ca(2+)-dependent manner. Prototypic members of this family are Ca(2+)-binding domains of calpain. Our recent finding that grancalcin interacts with L-plastin,(More)
In this study, we investigated the induction of apoptosis by ultrasound in the presence of a photochemically active chlorin, mono-l-aspartyl chlorin e6 (NPe6). HL-60 cells were exposed to ultrasound for up to 3 min in the presence and absence of NPe6, and the induction of apoptosis was examined by analyzing cell morphology, DNA fragmentation, and caspase-3(More)
AIM To investigate the pathophysiological role of platelet neuropeptide Y (NPY) in hypertension. METHODS The content of NPY in plasma and platelets from spontaneously hypertensive rats (SHR) and Wistar-Kyoto rats (WKR) were measured by radioimmunoassay. RESULTS There was no difference in NPY concentration between SHR plasma and WKR plasma (2.1 +/- 1.0(More)
Plasma neuropeptide Y (NPY) was measured by radioimmunoassay in 43 patients with congestive heart failure (CHF) and 20 healthy control subjects. The results showed that plasma NPY levels were elevated in patients with CHF compared with control subjects (366 +/- 28 ng/L VS. 89 +/- 22 ng/L, P < 0.001). There was a close relationship between the severity of(More)
The distribution of alpha 1-adrenoceptor and its subtypes in isolated aortae was compared between 12 month- and 10 week-old Wistar rats by determinating vasoconstrictor responses. In the 12 month-old rats, compared with the 10 week-old rats, (1) the maximal contraction induced by norepinephrine (NE) was reduced, without significant alteration of pD2 value;(More)
Recombinant human grancalcin, a calcium-binding protein from leukocytes, has been crystallized in the presence or absence of Ca(2+) by the vapor-diffusion method. Two crystal forms of apo grancalcin were obtained: space group P2(1), with unit-cell parameters a = 48.4, b = 81.1, c = 46.6 A, beta = 111.3 degrees, diffracting to 1.9 A, and space group C2, with(More)
AIM To characterize the subtype of alpha 1-adrenoceptor mediating vasoconstriction in perfused rat mesenteric vascular bed. METHODS The potencies (pA2 values determined by Schild plot) of alpha 1-adrenoceptor-selective antagonists were determined by isolated vasoconstrictive experiment. The pKi values were determined by 125I-BE 2254 binding from the(More)