Qing-Shan Li

Learn More
The metabolism of polychlorinated dibenzo-p-dioxins by cytochrome P450 BM-3 from Bacillus megaterium and a mutant enzyme of it (AL4V; Ala74Gly, Phe87Val, Leu188Gln triple mutant) was examined. Both purified enzymes metabolized 1-monochloro-, 2,3-dichloro-, and 2,3,7-trichloro-dibenzo-p-dioxin, but not 2,3,7,8-tetrachloro-dibenzo-p-dioxin. The mutant AL 4V(More)
Cytochrome P450 BM-3 from Bacillus megaterium is a fatty acid hydroxylase exhibiting selectivity for long-chain substrates (12–20 carbons). Replacement of Phe87 in P450 BM-3 by Val (F87V) greatly increased its activity towards a variety of aromatic and phenolic compounds. The apparent initial reaction rates of F87V as to benzothiophene, indan,(More)
A novel cytochrome c and a catalase-peroxidase with alkaline peroxidase activity were purified from the culture supernatant of Bacillus sp. No.13 and characterized. The cytochrome c exhibited absorption maxima at 408 nm (Soret band) in its oxidized state, and 550 (alpha-band), 521 (beta-band), and 415 (Soret band) nm in its reduced state. The native(More)
Cytochrome P450 BM-3 from Bacillus megaterium catalyzed NADPH-supported indole hydroxylation under alkaline conditions with homotropic cooperativity toward indole. The activity was also found with the support of H2O2, tert-butyl hydroperoxide (tBuOOH), or cumene hydroperoxide (CuOOH). Enhanced activity and heterotropic cooperativity were observed in(More)
In recent years, an increasing number of botnets use Domain Generation Algorithms (DGAs) to bypass botnet detection systems. DGAs, also referred as “domain fluxing”, has been used since 2004 for botnet controllers, and now become an emerging trend for malware. It can dynamically and frequently generate a large number of random domain names which are used to(More)
Bioprocesses, which involve biocatalysts for the production of useful compounds, are expected to become a leading player in green chemistry. The first step in bioprocess development is screening for useful biological reactions in the immense number of microorganisms with infinite diversity and versatility. This review introduces some examples of bioprocess(More)
In the supply chain, RFID tags are deployed more widely. In the life of the supply chain, the owner of the tag will change frequently. Ownership transfer protocol can achieve the purpose that the access rights of the tag are transferred from the original owner to the new owner, and protect the privacy of the original owner and the new owner. To resist(More)
The vagueness of queries is a thorny problem of web search all the time. Because of the length of queries is general short, identifying user's search intent from a single query session is unpractical. In this paper, a result recommendation method which refers to the idea of swarm intelligent to optimizing the result list is proposed to solve this problem.(More)
  • 1