Qin-xue Li

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OBJECTIVE To study the survival and developmental morphology of Parachlamydia (BN9) within Acanthamoeba. METHODS The morphology of BN9 within Acanthamoeba was studied by inverted phase contrast microscope, electron microscope, Gimenez and AO-staining with amoebal co-culture. RESULTS The endosomal maturation-blocked were formed after the egress of BN9.(More)
OBJECTIVE To determine whether Acanthamoeba polyphaga could affect the survival and growth of Vibrio cholerae O139 in low temperature. METHODS V. cholerae O139 was co-cultured with the Acanthamoeba polyphaga to be examined on its intracellular growth and survival rate within cysts at low temperature, using methods as Gram-staining, electron microscope and(More)
OBJECTIVE To establish a method for co-culture of amoebae and endosymbionts, also for continuously observing the microphenotype of amoebae. METHODS 24 wells culture plate with cover glass on the wells was used as containers. Amoebae and Candida albicans were co-cultured in microdrop of medium in the wells at 37 degrees C, and observed under x1000. (More)
OBJECTIVE To study the survival and growth of Vibrio cholerae inside the Acanthamoeba polyphage. METHODS Survival and growth of Vibro cholerae O139, co-cultured with Acanthamoeba polyphaga, was observed inside the trophozoites and cysts, using Gram stain and electron microscope. RESULTS Viable O139 was observed inside the amoebal vacuoles in 24 hours.(More)
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