Qin Si

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OBJECTIVE To study the changes of endogenous H2S and the effect of exogenously applied H2S on hypoxic pulmonary hypertension (HPH). METHODS Male Wistar rats were randomly divided into control group (n = 6), hypoxia group (n = 7) and hypoxia + NaHS group (n = 6). The rats that received hypoxia were put into a normobaric hypoxic chamber to respire hypoxic(More)
Platelet-derived growth factor-B (PDGF-B) is upregulated by proinflamatory stimuli in the early stages of atherosclerosis. However, its mechanisms are not fully elucidated. In the present study, by using the antioxidant N-acetylcysteine (NAC), we investigated in human umbilical vein endothelial cells (HUVECs) the roles of oxidative stress in PDGF-B(More)
The present study examined the effect and part mechanism of angiotensin II-stimulated integrin beta3 gene expression in human umbilical vein endothelial cells. Protein level and mRNA level of integrin beta3 expression were determined using enzyme-linked immunoadsorbent assay and reverse transcription-polymerase chain reaction. Four plasmids of 5'-different(More)
OBJECTIVE To investigate the effect of Ginkgo biloba extract (GBE) on acute lung injury(ALI) induced by lipopolysaccharide (LPS) in aging rats. METHODS The rats were randomly divided into two parts: six rats served as normal controls; 18 rats were used for producing the aging animal model ( D-gal 50 mg/kg body weight was injected intraperitoneally, once a(More)
The effects of sewage irrigation on the seedling growth of wheat and its activate oxygen metabolism were studied by water cultivation experiments. The results showed that compared with control, the wheat under sewage irrigation was short and small, and its roots number, fresh/dry weight of stem or root, and content of soluble protein decreased drastically.(More)
Result-chain based modeling is a new system analysis and process modeling method. This paper defines node influence degree (NID) to evaluate importance of nodes in a system (RCM model), so to help people to understand the system better. Both qualitative and quantitative evaluation method are described. In the qualitative evaluation method, out-degree and(More)
OBJECTIVE To investigate the effects of alpha-zearalanol (alpha-ZAL) on the generation of reactive oxygen species (ROS) and ROS-activated signal transduction in the tumor necrosis factor (TNF-alpha)-stimulated human umbilical vein endothelial cells (HUVECs). METHODS HUVECs were cultured and divided into 4 groups: (1) normal control group, (2) TNF-alpha(More)
OBJECTIVE To observe antiasthmatic and anti-inflammatory actions mechanisms of CDCA. METHOD The content of NO was determined by method of nitroreductase chromatometry in serum and trachea tissue. The content of cAMP was analysed by method of competitive protein-binding assay. The content of PGE2 was determined by method of ultraviolet spectrophotometry.(More)
To understand human papillomavirus (HPV) and expression of osteopontin (OPN) in cervical diseased tissues, HPV infection was detected in paraffin-embedded specimens of cervical lesions from 90 patients with cervical cancer. Three polymerase chain reaction (PCR) techniques were used to determine the detectable rate of HPV infection. Expression of HPV OPN(More)
OBJECTIVE To investigate the effect of alpha-zearalenol on angiotensin II-induced beta3 integrin mRNA expression in human umbilical vein endothelial cells (HUVECs). METHODS The mRNA level in integrin beta3 was determined by reverse transcription-polymerase chain reaction. Endothelial NF-kappaB activity was determined by the luciferase activity assay of(More)