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Epitope tagging is the insertion of a short stretch of amino acids constituting an epitope into another protein. Tagged proteins can be identified by Western, immunoprecipitation and immunofluorescence assays using pre-existing antibodies. We have designed vectors containing the URA3 gene flanked by direct repeats of epitope tags. We use the polymerase(More)
In Saccharomyces cerevisiae, three G1 cyclins (Clns) are important for Start, the event committing cells to division. Sic1, an inhibitor of C1b-Cdc28 kinases, became phosphorylated at Start, and this phosphorylation depended on the activity of Clns. Sic1 was subsequently lost, which depended on the activity of Clns and the ubiquitin-conjugating enzyme(More)
Spiral waves are a basic feature of excitable systems. Although such waves have been observed in a variety of biological systems, they have not been observed in the mammalian cortex during neuronal activity. Here, we report stable rotating spiral waves in rat neocortical slices visualized by voltage-sensitive dye imaging. Tissue from the occipital cortex(More)
It is common practice to use similar or even the same feature extraction methods for automatic speech recognition and speaker identification. While the front-end for the former requires to preserve phoneme discrimination and to compensate for speaker differences to some extend, the front-end for the latter has to preserve the unique characteristics of(More)
Received (received date) Revised (revised date) Communicated by Editor's name ABSTRACT An asymptotic lower bound for the maxrun function ρ(n) = max {number of runs in string x | all strings x of length n} is presented. More precisely, it is shown that for any ε > 0, (α−ε)n is an asymptotic lower bound, where α = 3 1+ √ 5 ≈ 0.927. A recent construction of an(More)
BACKGROUND Sepsis remains a major clinical challenge in intensive care units. The difficulty in developing new and more effective treatments for sepsis exemplifies our incomplete understanding of the underlying pathophysiology of it. One of the more widely used rodent models for studying polymicrobial sepsis is cecal ligation and puncture (CLP). While a(More)
In this paper a method for the automatic construction of a lexicon with multiple entries per word is described. The basic idea is to transform a reference word transcription by means of stochastic pronunciation rules that can be learned automatically. This approach already proved its potential (Cremelie & Martens, 1999), and is now brought to a much higher(More)
A simple, accurate and reliable method for the simultaneous separation and determination of 9 active components (danshensu, protocatechuic acid, protocatechuic aldehyde, caffeic acid, rosmarinic acid, salvianolic acid B, paeonol, paeoniflorin and gallic acid) in traditional Chinese medicinal preparation ShuangDan (SD) oral liquid was developed using reverse(More)