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Hairy root induction and benzylisoquinoline alkaloid production in Macleaya cordata
It is demonstrated that the hairy root system has further potential for bioengineering and sustainable production of sanguinarine on a commercial scale and the molecular mechanism that causes these metabolites to increase is revealed.
Comparative investigation of phytochemicals among ten citrus herbs by ultra high performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometry and
The medicine-food dual purpose herbs including Chenpi, Juluo, Daidaihua, Huajuhong, Xiangyuan and Foshou, exhibited antioxidant capacities significantly and were consistent with the multivariate analysis of their major flavonoids.
Chemical Profiles and Simultaneous Quantification of Aurantii fructus by Use of HPLC-Q-TOF-MS Combined with GC-MS and HPLC Methods
This study applied high-performance liquid chromatography to the identification of multiple bioactive substances and improve the quality control of Aurantii fructus, finding good linearity, precision, stability, repeatability and recovery.
N-(Acyloxy)phthalimides as tertiary alkyl radical precursors in the visible light photocatalyzed tandem radical cyclization of N-arylacrylamides to 3,3-dialkyl substituted oxindoles
A visible light promoted tandem radical cyclization of N-arylacrylamides with N-(acyloxy)phthalimides to 3,3-dialkyl substituted oxindoles was developed. In the presence of a photocatalyst
Tissue-specific metabolite profiling of benzylisoquinoline alkaloids in the root of Macleaya cordata by combining laser microdissection with ultra-high-performance liquid chromatography/tandem mass
The integrated method is sensitive, specific and reliable for determining trace alkaloids, which is also a powerful tool for metabolite profiling of tissue-specific BIAs in situ, and should contribute to a better understanding of the biosynthetic pathways in M. cordata.
Identification of gelsemine metabolites in rat liver S9 by high-performance liquid chromatography/quadrupole-time-of-flight mass spectrometry.
The present results showed that the metabolic pathways of gelsemine are oxidation, demethylation, and dehydrogenation in rat liver S9.