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A soluble α-synuclein construct forms a dynamic tetramer
TLDR
A dynamic model for the tetramer structure is proposed, based on expected close association of the amphipathic central helices observed in the previously described micelle-associated “hairpin” structure of α-synuclein, which indicates that a symmetric oligomer is present, with three- or fourfold symmetry. Expand
Bone recognition mechanism of porcine osteocalcin from crystal structure
TLDR
The X-ray crystal structure of porcine osteocalcin is presented at 2.0 Å resolution, which reveals a negatively charged protein surface that coordinates five calcium ions in a spatial orientation that is complementary to calcium ion in a hydroxyapatite crystal lattice. Expand
Structural basis for conformational plasticity of the Parkinson's disease-associated ubiquitin hydrolase UCH-L1.
TLDR
The three-dimensional structure of human UCH-L1 is determined at 2.4-A resolution by x-ray crystallography, and the overall fold resembles that of other ubiquitin hydrolases, including UCH- L3, but there are a number of significant differences. Expand
Structure and specificity of a quorum-quenching lactonase (AiiB) from Agrobacterium tumefaciens.
TLDR
This work reports the cloning, heterologous expression, purification, metal content, substrate specificity, and three-dimensional structure of AiiB, an AHL lactonase from Agrobacterium tumefaciens, which appears to be a metal-dependent AHL lact onase with broad specificity. Expand
Crystal structure of Bacillus subtilis GabR, an autorepressor and transcriptional activator of gabT
TLDR
The crystal structures reported here show that this protein has evolved from the fusion of a type I aminotransferase and a winged helix-turn-helix DNA-binding protein to form a chimeric protein that adopts a dimeric head-to-tail configuration, an example of a coenzyme playing a role in transcription regulation rather than in enzymatic catalysis. Expand
Caspase-1 causes truncation and aggregation of the Parkinson’s disease-associated protein α-synuclein
TLDR
It is reported that inflammation can play a role in causing the aggregation of wild type aSyn, and perhaps in the pathogenesis of sporadic PD as well. Expand
Parkinson disease-associated mutation R1441H in LRRK2 prolongs the “active state” of its GTPase domain
TLDR
It is shown that Roc forms a stable monomeric conformation in solution that is catalytically active, thus demonstrating that LRRK2 is a bona fide self-contained GTPase. Expand
Detection of ligand binding hot spots on protein surfaces via fragment-based methods: application to DJ-1 and glucocerebrosidase
TLDR
Comparison of data derived via MSCS and FTMap shows that FTMap, a computational method for the identification of fragment binding hot spots, is an accurate and robust alternative to the performance of expensive and difficult crystallographic experiments. Expand
Reducing C-terminal truncation mitigates synucleinopathy and neurodegeneration in a transgenic model of multiple system atrophy
TLDR
In vivo proof of concept of the ability of the caspase-1 inhibitor prodrug VX-765 to mitigate α-syn pathology and provide neuroprotection in a transgenic mouse model of MSA through reduction ofα-syn C-terminal truncation is presented. Expand
Hydrophilic Residues Are Crucial for Ribosomal Protein L11 (RPL11) Interaction with Zinc Finger Domain of MDM2 and p53 Protein Activation*
TLDR
The study reveals that RPL11 forms a stable complex with MDM2 in vitro through direct contact with its zinc finger through hydrophilic residues, providing the first line of evidence for the specific interaction between RPL 11 and the zinc finger of MDM 2 via hydrophobic residues. Expand
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