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Cre/lox System and PCR-Based Genome Engineering in Bacillus subtilis
ABSTRACT We have developed a fast and accurate method to engineer the Bacillus subtilis genome that involves fusing by PCR two flanking homology regions with an antibiotic resistance gene cassetteExpand
High-Level Expression and Secretion of Methyl Parathion Hydrolase in Bacillus subtilis WB800
ABSTRACT The methyl parathion hydrolase (MPH)-encoding gene mpd was placed under the control of the P43 promoter and Bacillus subtilis nprB signal peptide-encoding sequence. High-level expression andExpand
Biodegradation of acetamiprid by Pigmentiphaga sp. D-2 and the degradation pathway
Abstract Acetamiprid is a widely-used neonicotinoid insecticide often detected in the environment. In this study, we aimed to isolate high-efficient acetamiprid-degrading strains and to investigateExpand
mazF, a novel counter-selectable marker for unmarked chromosomal manipulation in Bacillus subtilis
Here, we present a novel method for the directed genetic manipulation of the Bacillus subtilis chromosome free of any selection marker. Our new approach employed the Escherichia coli toxin gene mazFExpand
Isolation of Fenitrothion-degrading Strain Burkholderia sp. FDS-1 and Cloning of mpd Gene
A short rod shaped, gram-negative bacterium strain Burkholderia sp. FDS-1 was isolated from the sludge of the wastewater treating system of an organophosphorus pesticides manufacturer. The isolateExpand
Biodegradation of chlorpyrifos and 3,5,6-trichloro-2-pyridinol by Cupriavidus sp. DT-1.
A bacterial strain, Cupriavidus sp. DT-1, capable of degrading chlorpyrifos and 3,5,6-trichloro-2-pyridinol (TCP) and using these compounds as sole carbon source was isolated and characterized.Expand
Burkholderia zhejiangensis sp. nov., a methyl-parathion-degrading bacterium isolated from a wastewater-treatment system.
The taxonomic status of a methyl-parathion-degrading strain, OP-1(T), isolated from a wastewater-treatment system in China, was determined using a polyphasic approach. The rod-shaped cells wereExpand
Isolation and characterization of a carbofuran-degrading strain Novosphingobium sp. FND-3.
A gram-negative Novosphingobium sp. strain FND-3 capable of degrading carbofuran was isolated and characterized. The carbofuran-degrading ability of strain FND-3 was investigated under variousExpand
Characterization of phenol-degrading bacterial strains isolated from natural soil
Abstract Six phenol-degrading bacteria designated as PND-1–PND-6 were isolated from natural soil. PND-1–PND-6 were from the genera of Pseudomonas , Acinetobacter , Comamonas and Cupriavidus ,Expand
Rhizobium petrolearium sp. nov., isolated from oil-contaminated soil.
Two Gram-negative, aerobic, rod-shaped bacteria, designated strains SL-1(T) and F11, which had the ability to decompose polycyclic aromatic hydrocarbons (PAHs), were isolated from soil samplesExpand
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