Prudence Talbot

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Fertilized mouse eggs regulate their size principally by accumulating glycine as an intracellular osmolyte using the GLYT1 (SLC6A9) transporter, a mechanism of cell volume homeostasis apparently unique to early embryos before the morula stage. However, nothing was known of cell volume regulation in oocytes before fertilization. We show here that GLYT1 is(More)
Fertilization in mammals requires the successful completion of many steps, starting with the transport of gametes in the reproductive tract and ending with sperm-egg membrane fusion. In this minireview, we focus on three adhesion steps in this multistep process. The first is oocyte "pick-up," in which the degree of adhesion between the extracellular matrix(More)
Mammalian sperm must pass between cumulus cells and corona radiata cells before reaching the surface of the zona pellucida which surrounds the oocyte. The cumulus and corona radiata cells are separated from each other by an extracellular matrix (ECM) containing hyaluronic acid. The structure of this ECM and of the zona pellucida was investigated in the(More)
We have developed a simple direct method, requiring minimal manipulation, to measure beat frequencies of the cilia on the external surface of hamster oviducal infundibula in vitro. Two perfusion chambers (closed and open) were used; both can be hand-made in a few minutes and discarded after use. Ciliary beat frequencies were determined by measuring(More)
— This paper proposes an automated detection method with simple algorithm for detecting human embryonic stem cell (hESC) regions in phase contrast images. The algorithm uses both the spatial information as well as the intensity distribution for cell region detection. The method is modeled as a mixture of two Gaussians; hESC and substrate regions. The paper(More)
—Human Embryonic Stem Cells (HESCs) possess the potential to provide treatments for cancer, Parkinson's disease, Huntington's disease, Type 1 diabetes mellitus etc. Consequently , HESCs are often used in the biological assay to study the effects of chemical agents in the human body. However, detection of HESC is often a challenge in phase contrast images.(More)
Secondhand smoke (SHS) is a confirmed lung carcinogen that introduces thousands of toxic chemicals into the lungs. SHS contains chemicals that have been implicated in causing oxidative DNA damage in the airway epithelium. Although DNA repair is considered a key defensive mechanism against various environmental attacks, such as cigarette smoking, the(More)
Human Embryonic Stem Cells (HESCs) are promising for the treatment of many diseases and for toxicological testing. There is a great interest among biologists to automatically determine the number of various types of cells in a population of mixed morphologies. This study addresses quantification of non-dynamic blebbing single unattached human embryonic stem(More)
In this paper, we propose an automatic method to detect human embryonic stem cell regions. The proposed method utilizes the K-means algorithm with weighted entropy. As in phase contrast images the cell regions have high intensity variation, they usually yield higher entropy values than the substrate regions which have less intensity variation. Thus, the(More)