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The norpAH44 phototransduction mutant of Drosophila melanogaster, an allele that, on eclosion, does not exhibit a receptor potential was found, at later ages, to undergo light and temperature dependent degeneration of its photoreceptors as well as decreases in rhodopsin concentration. Pseudopupil measurements and light and electron microscopy were used to(More)
The physiological regulation of light-activated cyclic GMP phosphodiesterase (EC in rod outer segments has been shown to depend upon a heat-stable inhibitor and upon the reversal of its effect by a specific GTP/GTP-binding protein complex (Hurley, J. B. (1980) Biochem. Biophys. Res. Commun. 92, 505-510; Yamazaki, A., Bartucca, F., Ting, A., and(More)
Light-induced activation of cyclic GMP phosphodiesterase (PDE) in the outer segments of vertebrate rod photoreceptors has been suggested as a step in the transduction process linking the absorption of light by rhodopsin with a hyperpolarization of the plasma membrane. Activation of PDE is mediated by a 'GTP-binding protein' (G). As a result of interaction(More)
Mosquito rhodopsin is a digitonin-soluble membrane protein of molecular weight 39,000 daltons, as determined by sodium dodecyl sulfate gel electrophoresis. The rhodopsin undergoes a spectral transition from R515-520 to M480 after orange illumination. The visual pigment apoprotein, opsin, is the major membrane protein in the eye. Protein synthesis in the(More)
Der Begriff „Adenokarzinom des ösophagogastralen Übergangs“ (AEG) umfasst sowohl aus klinischer wie auch aus tumorbiologischer Sicht unterschiedliche Tumorentitäten. Die rein anatomisch-topographische Klassifikation der AEGs in Adenokarzinome des distalen Ösophagus (AEG I), eigentliche Kardiakarzinome (AEG II) und subkardiale Magenkarzinome (AEG III) trägt(More)
Five GTP binding proteins in rat cerebral cortex synaptic membranes were identified by photoaffinity labelling with [3H] or [32P](P3-azido-anilido)-P1-5' GTP (AAGTP). When AAGTP-treated membranes were incubated with colchicine or vinblastine and subsequently washed, a single AAGTP-labelled protein of 42 kD was released into the supernatant. About 30% of the(More)
In this report, we show that fluoride activates dark-adapted rod outer segment phosphodiesterase, and that this activation is mediated, in analogy with adenylate cyclase, through a GTP binding protein. The GTP binding protein is released from dark-adapted rod outer segment membranes by exposure to fluoride and subsequent centrifugation. The 39-kilodalton(More)
PURPOSE To determine whether a new cyclic GMP analog, 2'-O-(4-benzoyl)benzoylguanosine 3',5' cyclic monophosphate (BB-cGMP), significantly reduces intraocular pressure in either normotensive or hypertensive eyes of rabbits. METHODS Intraocular pressure of normal adult rabbits and/or rabbits with alpha-chymotrypsin-induced ocular hypertension was measured(More)