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An underlying mechanism for multi drug resistance (MDR) is up-regulation of the transmembrane ATP-binding cassette (ABC) transporter proteins. ABC transporters also determine the general fate and effect of pharmaceutical agents in the body. The three major types of ABC transporters are MDR1 (P-gp, P-glycoprotein, ABCB1), MRP1/2 (ABCC1/2) and BCRP/MXR(More)
application notes imaging and fluorescence exposed Jurkat cells to 0.1 µg ml –1 nocodazole, 3 µM etoposide or 30 µg ml –1 aphidicolin. Then we incubated the cells with a 1:250 dilution of Nuclear-ID Red DNA stain in medium and assayed the cultures using the FL3 channel of a FACSCalibur flow cytometer (BD Biosciences), equipped with a 488-nm laser. Flow(More)
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