Pradeep K. Malakar

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We have produced data and developed analysis to build representations for the concentration of spores of nonproteolytic Clostridium botulinum in materials that are used during the manufacture of minimally processed chilled foods in the United Kingdom. Food materials are categorized into homogenous groups which include meat, fish, shellfish, cereals, fresh(More)
Network models offer computationally efficient tools for estimating the variability of single-cell lag phases. Currently, optical methods for estimating the variability of single-cell lag phases use single-cell inocula and are technically challenging. A Bayesian network model incorporating small uncertain inocula addresses these limitations.
A novel TaqMan-based multiplex real-time PCR method combined with propidium monoazide (PMA) treatment was firstly developed for the simultaneous quantification of viable Vibrio parahaemolyticus and Listeria monocytogenes in raw shrimp. The optimization of PMA concentration showed that 100 μM was considered optimal to effectively inhibit 108 CFU/mL dead(More)
Botulinum neurotoxins (BoNTs) produced by the anaerobic bacterium Clostridium botulinum are the most potent biological substances known to mankind. BoNTs are the agents responsible for botulism, a rare condition affecting the neuromuscular junction and causing a spectrum of diseases ranging from mild cranial nerve palsies to acute respiratory failure and(More)
Vibrio parahaemolyticus is the leading cause of seafood-derived illness in China and a possible mechanism leading to illness is cross contamination of cooked shrimp. A growth rate model of pandemic V. parahaemolyticus O3:K6 strain on cooked shrimp stored at isothermal temperatures was developed and validated. The maximum specific growth rate of this(More)
Risk from an uncertain small inoculum depends on variability of single-cell lag times. However, quantifying single-cell variability is technically challenging. It is possible to estimate this variability using population growth parameters. We demonstrate this possibility using data from literature and show a Bayesian scheme for performing this task.
The estimated public health burden of Campylobacter infection and illness in England and Wales due to consuming undercooked poultry products is relatively small, ~0.3 percent. The estimated annual number of cases of campy-lobacteriosis in England and Wales attributed to consumption of chicken is 175,000. Information, on consumer behavior during cooking,(More)
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