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Using a fluorescent viability assay, immunocytochemistry, patch-clamp recordings, and Ca(2+) imaging analysis, we report that ouabain, a specific ligand of the Na(+),K(+)-ATPase cardiac glycoside binding site, can prevent glutamate receptor agonist-induced apoptosis in cultured rat cortical neurons. In our model of excitotoxicity, a 240-min exposure to 30(More)
Recent studies suggested contribution of homocysteine (HCY) to neurodegenerative disorders and migraine. However, HCY effect in the nociceptive system is essentially unknown. To explore the mechanism of HCY action, we studied short- and long-term effects of this amino acid on rat peripheral and central neurons. HCY induced intracellular Ca²⁺ transients in(More)
Human endometrium-derived mesenchymal stem cells (hMESCs) enter the premature senescence under sublethal oxidative stress, however underlying mechanism remains unknown. Here, we showed that exogenous H2O2 induces a rapid phosphorylation and co-localization of ATM, H2A.X, 53BP1 leading to DNA damage response (DDR) activation. DDR was accompanied with nuclear(More)
Whereas kainate (KA)-induced neurodegeneration has been intensively investigated, the contribution of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors (AMPARs) in neuronal Ca2+ overload ([Ca2+]i) is still controversial. Using Ca2+ imaging and patch-clamp techniques, we found different types of Ca2+ entry in cultured rat cortical neurons. The(More)
92 1 Ionotropic glutamate receptors (GluR) in the verr tebrate central nervous system [1] are the main subject of interest while discussing " excitotoxicity " or neuroo toxic action of glutamate (Glu) [2–4], that usually can be revealed during an accumulation of excitatory neuu rotransmitter in the extracellular space due to its release from neurons and(More)
Effects of 0.01 nM–1 nM ouabain on neuronal survival in excitotoxic stress and ouabain self toxic action in concentrations from 10 nM to 30 μM were studied. Neuronal viability was evaluated by measuring Bcl-2 protein expression and using vital staining test allowing recognition of live, necrotic and apoptotic cells. Excitotoxic stress was induced by 240-min(More)
To evaluate the possible role of the plasma membrane Na(+)/Ca(2+)-exchanger (NCX) in regulation of N-methyl-d-aspartate (NMDA) receptors (NMDARs), we studied effects of 2-[2-[4-(4-nitrobenzyloxy) phenyl]ethyl]isothiourea methanesulfonate (KB-R7943; KBR) and lithium (inhibitors of NCX) on NMDA-elicited whole-cell currents using the patch-clamp technique on(More)
Here we show that in primary culture of rat cortical neurons the number of episodes of epileptiform curents (EC) provoked by extracellular magnesium removal increases over time. We demonstrate that NMDA receptor agonists in low concentrations induce an elevation of frequency of miniature postsynaptic currents followed by their synchronization resulting in(More)
Homocysteine, a sulfur-containing amino acid, exerts neurotoxic effects and is involved in the pathogenesis of many neurodegenerative disorders. In contrast to well-studied glutamate excitotoxicity, the mechanism of homocysteine neurotoxicity is not clearly understood. Using wholecell patch-clamp, calcium imaging (fluo-3) and measurements of mitochondrial(More)
Small-conductance calcium-activated potassium channels (SK channels) are widely expressed in CNS tissues. Their functions, however, have not been well studied. Participation of SK channels in Purkinje cell (PC) pacemaker activity has been studied predominantly in vitro. Here we studied for the first time the effects of SK channel activation by NS309 or(More)