Piotr Makowski

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The paper presents an active-contour segmentation method for 2D structures in MR images. The method combines two approaches to active contour segmentation, known as balloons and snakes. This makes the method shape independent and accurate. New anti-tangling features were introduced to improve segmentation of very complex object shapes, e.g. the left(More)
A novel approach to three-dimensional (3D) visualization of high quality, respiratory compensated cardiac magnetic resonance (MR) data is presented with the purpose of assisting the cardiovascular surgeon and the invasive cardiologist in the pre-operative planning. Developments included: (1) optimization of 3D, MR scan protocols; (2) dedicated segmentation(More)
Trans-4-hydroxy-2-nonenal (4-HNE), a cytotoxic end product of lipid peroxidation, is present in normal human blood plasma at concentrations of 0.1-1.0 microM. It can be, however, further metabolized within a cell, and one of the main products is 4-HNE glutathione conjugate (HNE-SG). In human erythrocyte membrane the system for active extrusion of(More)
With the use of ATP analogues, we have found that porcine liver annexin (Anx) IV can be covalently labelled with 8-azido[gamma-32P]-ATP in the presence of Ca2+ (Kd 4.2 microM) and that the labelling is prevented by asolectin/cholesterol liposomes or chelation of calcium ions. On the other hand, non-covalent binding of 2'-(or(More)
Purpose To develop a model independent method for heart and blood vessel segmentation in cardiac MR images to be used for 3D visualization on a virtual reality device. Introduction The aim was to be able to segment cardiac and vascular structures in dynamic multislice, multiphase short axis images obtained with breath-held ECG-triggered gradient echo EPI as(More)
We have previously demonstrated by tryptophan fluorescence the interaction of caldesmon with anionic phospholipid vesicles [Czurylo, Zborowski and Dabrowska (1993) Biochem. J. 291, 403-408]. In the present work we investigated the interaction of caldesmon with natural-membrane (rat liver endoplasmic reticulum) phospholipids by co-sedimentation assay. The(More)
The guanidine derivative U-37883A has been found to stimulate in vitro synthesis of phosphatidylserine in endoplasmic reticulum membranes, catalyzed exclusively by a serine-specific base exchange enzyme. The stimulation of the enzyme activity by the drug was concentration-dependent, with EC50 of 54 microM, while the biologically inactive analog of U-37883A,(More)
Properties of Ca2+-stimulated incorporation of amincalcohols, serine and ethanolamine, into phospholipids, and factors regulating the reaction were studied in endoplasmic reticulum membranes isolated from rat liver. In contrast to apparent Km values for either aminoalcohol, maximal velocities of the reaction were significantly affected by Ca2+(More)
A coherence-based description of the Lyot depolarizer illuminated by polychromatic light of any spectral density distribution is proposed as a generalization of the formulas provided for symmetrical spectra by Burns [J. Lightwave Technol. 1, 475 (1983)] and Mochizuki [Appl. Opt. 23, 3284 (1984)]. The structure of the derived expressions is explained in(More)