Pin-Yi Wu

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The structure and T cell stimulatory effects of the recently discovered cytokine IL-23 are similar to, but distinct from, those of IL-12. Although the antitumor activities of IL-12 are well characterized, the effect of IL-23 on tumor growth is not known. In this study, murine CT26 colon adenocarcinoma and B16F1 melanoma cells were engineered using(More)
In vivo electroporation (EP) of the murine interleukin-12 (IL-12) gene in an expression plasmid (pIL-12) was evaluated for antitumor activity. EP transfer of pIL-12 into mouse quadriceps muscles elicited significant levels of serum IL-12 and interferon-gamma. Intramuscular EP of pIL-12 resulted in complete regression or substantial inhibition of 38C13(More)
Identification of the cytotoxic T lymphocyte (CTL) epitopes of tumor antigens is important for effective immunotherapy. We report that a combination of epitope prediction, enzyme-linked immunosorbent assay (ELISA)-based epitope-HLA complex formation, and DNA immunization methods can improve the efficiency and accuracy of CTL epitope studies. In this study,(More)
Using a hepatitis B virus (HBV) transgenic mouse model, we previously showed that a single dose of double-stranded adeno-associated virus (dsAAV) vector serotype 8 carrying a small hairpin RNA (shRNA) effectively reduces HBV replication and gene expression, but the effect gradually decreases with time. In this report, we compared the anti-HBV RNA(More)
In order to determine the mechanism of generation of immunological memory, we used the splenic focus assay to analyze primary and in vitro generated secondary phosphorylcholine (PC)-specific B cell precursors in CBA/CaHN-xid/J mice (CBA/N) expressing an X-linked immune defect (xid). A quantitative analysis of the kinetics of the appearance of these(More)
To better understand the regulation of Ig repertoire expression and the generation of memory B cells, we have used the splenic focus assay to analyze primary and in vitro generated secondary phosphorylcholine (PC)-specific BALB/c B cell precursors. A quantitative analysis of the kinetics of appearance of these precursors over the time of the culture period(More)
In this paper, a concentration evaluation of reading behaviors with electrical signal detection on the head is presented. The electrode signal is extracted by brain-computer-interface (BCI) to monitor the user's degree of concentration, where the user is reminded by sound to concentrate, or teaching staffs are reminded to help users improve reading habits,(More)
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