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A cosmid clone containing the entire human argininosuccinate lyase gene was isolated and characterized. The gene is approximately 35 kb in length and is divided into 16 exons. The sequence surrounding all splice sites was determined, and one unusual 5' splice site sequence in which a GC dinucleotide occurred instead of the consensus GT dinucleotide was(More)
1.1 Theoretical overview The analysis of Serbo-Croatian (SC) second position clitic placement has been the source of much controversy in generative linguistics. The most important point of disagreement among competing analyses of this phenomenon has been the extent to which the various components of the grammar—syntax, morphology, phonology—are implicated(More)
Argininosuccinic aciduria is an inborn error of metabolism due to the genetic deficiency of argininosuccinate lyase. In order to determine the molecular basis for the disease, RNA isolated from cultured skin fibroblasts derived from four unrelated patients was reverse-transcribed and amplified using the polymerase chain reaction and the products were cloned(More)
The major soluble protein in the lenses of most birds and reptiles is delta-crystallin. In chickens and ducks the delta-crystallin gene has duplicated, and in the duck both genes contribute to the protein in the lens, while in the chicken lens there is a great preponderance of the delta 1 gene product. Purified delta-crystallin has previously been shown to(More)
The duck delta 2-crystallin gene encodes an enzymatically-active argininosuccinate lyase while the delta 1-crystallin gene product, although 94% identical, is enzymatically inactive. Four histidine residues in the duck delta 2-crystallin. His91, His110, His162 and His178, were converted to asparagine residues in an effort to define the role of histidines in(More)
Natural fires are an integral part of Mediterranean ecosystems. However, in Europe, the extensive use of natural and forest regions as recreational areas has increased the number of human caused fires. Additionally, the decrease of rural population and abandonment of agricultural regions has led to the build up of fire fuels on these areas and the(More)
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