Pieter Bouma

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We studied spatial niche metrics of large-bodied newts (Triturus cristatus and T. marmoratus) in three breeding ponds in western France. Adults and larvae were sampled with underwater funnel traps. Larvae were identified to the species with diagnostic microsatellite DNA markers. The distribution of adult T. cristatus and T. marmoratus across pond regions(More)
In isolated hearts perfused with salt solutions, albumin is often added in low concentrations for preservation of endothelial function. Albumin in low concentrations is also sometimes used as an intravascular indicator in such preparations. We perfused isolated rabbit hearts according to Langendorff with a constant flow of Tyrode solution. Despite maximal(More)
We studied four affected individuals from a family of three generations with Ehlers-Danlos Syndrome II. Type V collagen transcripts of affected individuals were screened by reverse transcriptase-polymerase chain reaction. Amplification of the exon 9-28 region of alpha1(V) yielded normal and larger products from the proband. Sequencing of cDNA revealed a(More)
We studied the effect of cardiac contraction on coronary arterial flow with vasomotor tone (control) and during maximal vasodilation with adenosine in the isolated blood-perfused rat heart at constant perfusion pressure (105 mmHg) with a left ventricular balloon (n = 6). Ventricular pressure was changed by volume changes and contractility via(More)
In experiments reported in the literature, oxygen uptake in saline-perfused heart decreased after small reductions in arterial O2 concentration (CaO2) at constant perfusion flow. This may have resulted from the decrease in O2 supply, but may also have been due to decreased O2 demand caused by reduced perfusion pressure following hypoxic vasodilation (garden(More)
Many vasoactive substances are involved in the regulation of vasomotor tone and some of them, like nitric oxide (NO), are derived from the endothelium. Nitric oxide is able to relax preconstricted coronary resistance vessels almost completely. However, it is not clear what the contribution of NO is to vasomotor tone in the intact blood perfused heart. The(More)
To improve the appropriateness and efficiency of diagnostic serological tests and subsequent antibiotic treatment, clinical data from 102 patients with unclassified arthritis were analysed to investigate whether the presence of positive IgG antibodies to Borrelia burgdorferi could be predicted. The clinical data were blindly ranked from 1 to 4 (1, Lyme(More)
During cardiac contraction coronary arterial inflow is impeded, whereas venous flow is augmented. These effects are assumed to be caused by diameter reductions of intramyocardial blood vessels. The reduction in vascular diameter (and thus vascular volume) during contraction increases coronary resistance and/or decreases back pressure so that flow decreases(More)
The correlation between blood levels of tricyclic antidepressants and clinical effect is still conflicting. Nevertheless in toxicology, clinical pharmacy and pharmacology a rapid, selective and sensitive assay for these drugs and their metabolites is needed. AHplc-method is presented for the rapid determination of amitriptyline, nortriptyline, clomipramine,(More)