Pierre L'Espérance

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Granulocyte colonies can be grown from leukocyte-rich plasma obtained from normal human peripheral blood. Purification of lymphocytes by the Ficoll-Hypaque gradient method and subsequent removal of monocytes achieved a 10-fold increase in the number of colonies formed, suggesting that the colony-forming cell is nonadherent, nonphagocytic, and(More)
A patient with severe combined immunodeficiency received seven transplants of bone marrow from an HLA-B-compatible and HLA-D-compatible unrelated donor in an attempt to provide immunologic reconstitution. The first four transplants achieved restricted engraftment with evidence of rudimentary immunologic function. A fifth transplant, given after low-dose(More)
Congenital neutropenia is a lethal disease characterized by recurrent infections beginning in the neonatal period, absence of neutrophils in the peripheral blood, eosinophilia, and monocytosis. The bone marrow shows an apparent "maturation arrest" of the neutrophil series at the promyelocyte stage. Granulocytic colonies grown in vitro in soft agar medium(More)
Congenital neutropenia (CN), a disease characterized by recurrent infections leading to death in infancy, shows a maturation arrest of the myeloid series at the promyelocyte-myelocyte level. The potential value of marrow transplantation in this disease would be determined by the nature of the underlying defect. However, studies to date have failed to define(More)
Children with congenital neutropenia (Kostman disease) present a syndrome of recurrent and eventually fatal bacterial infections. The disease is uniformly lethal and the mean age at death is 24 months. To attempt to better understand the nature of this inborn error, we have studied marrow and peripheral blood of 3 children with the disease. Along with(More)
This paper deseribes a simulation model representing the annealing operations in a rolling-mill facility. The model was developed using the discrete event simulation language SIMAN. The model integrates a friendly user interface through which the user interacts with the simulation model without programming or recompiling. The simulation model also(More)