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Journals and Conferences
We constructed three recombinant vectors derived from the herpes simplex virus type 1 mutant tsK, each of which contained a different transgene under the control of the herpes simplex virus type 1 immediate early 3 promoter inserted into the thymidine kinase locus: the prokaryotic enzymes beta-galactosidase and chloramphenicol acetyl transferase, and a… (More)
The cis-acting signals required for cleavage and encapsidation of the herpes simplex virus type 1 genome lie within the terminally redundant region or a sequence. The a sequence is flanked by short direct repeats (DR1) containing the site of cleavage, and quasi-unique regions, Uc and Ub, occupy positions adjacent to the genomic L and S termini,… (More)
We have used total internal reflection fluorescence (TIRF) to measure the adsorption kinetics of a newly synthesized fluorescent derivative of a triblock copolymer comprising two poly(ethylene oxide) arms connected by a poly(butylene oxide) segment. The composition is (EO)400 (BO)55 (EO)400, in which EO represents ethylene oxide, BO represents butylene… (More)
This report describes the two-dimensional rectification of STIS spectroscopic data performed by calstis7 in the STIS calibration pipeline. Two-dimensional rectification produces a flux-calibrated, rectified image with distance along the slit running linearly along the y axis and dispersion running linearly along the x axis.
The Cosmic Origins Spectrograph (COS) was installed on the Hubble Space Telescope (HST) in May 2009 as part of the most recent Servicing Mission 4. COS is a fourth-generation instrument that has significantly extended HST UV spectroscopic capabilities. This paper highlights the current instrument performance, with particular emphasis on the initial on-orbit… (More)
Procorticotrophin-releasing hormone (ProCRH) is the precursor to the hypothalamic neuropeptide CRH(1-41) which mediates the neuroendocrine response to stress. In neuroendocrine cells and neurones, peptide hormones and neuropeptides are targeted to the dense-core vesicles of the regulated secretory pathway. These vesicles are transported to the ends of the… (More)
To investigate the intracellular localisation and biological activity of procorticotrophin-releasing hormone (proCRH), we have established stably transfected CHO-K1 cells expressing the rat pre-proCRH cDNA. Using immunoblot analysis of cell lysates of transfected CHO-K1 cells, we detected a major CRH immunoreactive band with an apparent molecular weight of… (More)
STIS data can be taken as a series of identical exposures, the so-called REPEATOBS mode. We describe the procedure for combining these data in the STIS Pipeline for different instrument configurations. Purposes and shortcomings of the followed procedure are identified.