Ph Rousselot

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By using specific antibodies and radioimmunological and immunohistochemical methods, we here show that neuropeptide FF (NPFF) occurs in cervical and lumbar dorsal root ganglia cells. Levels in the ganglia were low because they were detectable only after colchicine treatment or after unilateral dorsal rhizotomy. Similar high-performance liquid chromatography(More)
Pre- and postembedding immunocytochemical procedures were used, together with antisera raised against oxytocin or its neurophysin, to characterize oxytocinergic pathways in the rat spinal cord, at the electron microscopic level. Pre-embedding immunoperoxidase staining performed on vibratome sections revealed oxytocin- and neurophysin-positive axonal(More)
The bovine octapeptide Phe-Leu-Phe-Gln-Pro-Gln-Arg-Phe-NH2 (FLFQPQRFamide), originally detected by antisera raised against the invertebrate peptide, Phe-Met-Arg-Phe-NH2, is a neuropeptide which antagonizes the actions of endogenous and exogenous opiates. Using a sensitive radioreceptor assay, we show that rat spinal cord extracts were able to inhibit(More)
We used transganglionic transport of the neuronal tracer horseradish peroxidase coupled to wheat germ agglutinin (HRP-WGA) and post-embedding immunogold staining to determine the spinal projections and neurochemical identity of sensory afferents originating from a discrete cutaneous area. After SC injection of tracer into the nipple of lactating rats and(More)
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