Petri Pulkkinen

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Scots pine (Pinus sylvestris) genomic libraries were constructed and screened with oligonucleotides probes (GT)10, (CT)10, and (AT)10. Eight microsatellites were identified from 6000 clones screened. The longest microsatellite stretch found, (CT)9(N)21(AT)24, was amplified from bud and single pollen grain samples. In order to clarify the complex(More)
We investigated the functional and structural responses of Scots pine to climate and estimated the importance of the genotype on the traits studied. We analysed 13C isotope discrimination (Δ13C) of various provenances in a common garden experiment and gas exchange characteristics for provenances growing in their natural environment. No clear climatic trend(More)
We studied whether the differences between genetically different pollen donors (Betula pendula Roth clones) with respect to pollen-tube growth rate were consistent under different thermal conditions during pollen germination in vivo and in vitro. We conducted a single-donor hand-pollination experiment with same pollen donors and recipients in a plastic(More)
1. Diamine oxidase [amine-oxygen oxidoreductase (deaminating)(pyridoxal-containing), EC 1.4.3.6] was purified from human seminal plasma more than 1,700-fold. The enzyme appeared to be homogeneous on polyacrylamide-gel electrophoresis at two different pH values. 2. The general properties of the enzyme were comparable with those described for other diamine(More)
Human spermatozoa, separated from the seminal plasma, contained substantial amounts of spermine, and also a distinct diamine oxidase (EC1.4.3.6) activity. Spermine, but not diamine oxidase activity, could be removed from the spermatozoa by washing the cells with buffers containing high concentrations of salts. Incubation of human spermatozoa in the presence(More)
The relationship between pollen and progeny performance has been a subject of many studies but the evidence for pollen-tube growth rate as an indicator of progeny fitness is equivocal. We used an anemophilous tree, Betula pendula, to examine the relationship between pollen-tube growth rate and seed and seedling performance. We crossed nine maternal plants(More)
Although various complex, bulky ligands have been used to functionalize plasmonic gold nanoparticles, introducing them to small, atomically precise gold clusters is not trivial. Here, we demonstrate a simple one-pot procedure to synthesize fluorescent magic number Au25 clusters carrying controlled amounts of bulky calix[4]arene functionalities. These(More)
In this study, surface coatings were used to control the morphology of the deposited lipid layers during vesicle spreading, i.e., to control if liposomes self-assemble on a surface into a supported lipid bilayer or a supported vesicular layer. The influence of the properties of the surface coating on formation of the deposited lipid layer was studied with(More)
Gold nanoparticles were synthesized and inkjet-printed on a paper substrate and IR-sintered to produce conductive electrodes. The electrodes were further functionalised by using self-assembled octadecanethiol monolayers (SAMs). The effect of sintering, print quality, and SAM formation were examined by topographical, chemical and electrical methods. With(More)
There is an urgent need for accessible purification and separation strategies of atomically precise metal clusters in order to promote the study of their fundamental properties. Although the separation of mixtures of atomically precise gold clusters Au25L18, where L are thiolates, has been demonstrated by advanced separation techniques, we present here the(More)