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Scots pine (Pinus sylvestris) genomic libraries were constructed and screened with oligonucleotides probes (GT)10, (CT)10, and (AT)10. Eight microsatellites were identified from 6000 clones screened. The longest microsatellite stretch found, (CT)9(N)21(AT)24, was amplified from bud and single pollen grain samples. In order to clarify the complex(More)
1. Diamine oxidase [amine-oxygen oxidoreductase (deaminating)(pyridoxal-containing), EC 1.4.3.6] was purified from human seminal plasma more than 1,700-fold. The enzyme appeared to be homogeneous on polyacrylamide-gel electrophoresis at two different pH values. 2. The general properties of the enzyme were comparable with those described for other diamine(More)
In this study, surface coatings were used to control the morphology of the deposited lipid layers during vesicle spreading, i.e., to control if liposomes self-assemble on a surface into a supported lipid bilayer or a supported vesicular layer. The influence of the properties of the surface coating on formation of the deposited lipid layer was studied with(More)
Human spermatozoa, separated from the seminal plasma, contained substantial amounts of spermine, and also a distinct diamine oxidase (EC1.4.3.6) activity. Spermine, but not diamine oxidase activity, could be removed from the spermatozoa by washing the cells with buffers containing high concentrations of salts. Incubation of human spermatozoa in the presence(More)
Gold nanoparticles were synthesized and inkjet-printed on a paper substrate and IR-sintered to produce conductive electrodes. The electrodes were further functionalised by using self-assembled octadecanethiol monolayers (SAMs). The effect of sintering, print quality, and SAM formation were examined by topographical, chemical and electrical methods. With(More)
Although various complex, bulky ligands have been used to functionalize plasmonic gold nanoparticles, introducing them to small, atomically precise gold clusters is not trivial. Here, we demonstrate a simple one-pot procedure to synthesize fluorescent magic number Au25 clusters carrying controlled amounts of bulky calix[4]arene functionalities. These(More)
The aim of this research was to explore the use of amine-containing polymeric and low-molar-mass organic protecting agents in the preparation of copper nanoparticles. Particles were synthesized using poly(ethylene imine) (PEI) or tetraethylenepentamine (TEPA) as protecting agents. The resulting particles were studied with UV-vis spectrometry,(More)
There is an urgent need for accessible purification and separation strategies of atomically precise metal clusters in order to promote the study of their fundamental properties. Although the separation of mixtures of atomically precise gold clusters Au25L18, where L are thiolates, has been demonstrated by advanced separation techniques, we present here the(More)
Oxidized spermine, an iminoaldehyde (N,N'-bis (3-propionaldehyde) 1,4-diaminobutane), is a non-competitive inhibitor of fructolysis by human spermatozoa. The inhibition constant is about 0.3 mM. In experiments with [U-14C]fructose the iminoaldehyde caused a more pronounced depression of the formation of CO2 than of lactate. The iminoaldehyde was without(More)
A novel process for identifying reusable program clones is described in this work. The process is applied to a very demanding context, a large-scale closed source healthcare software system. As results, the applicability and maturity of the clone detection system are discussed but also the results of the study are summarized. In this, especial care has been(More)