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The 23.5 kd protein product of the ras-related YPT1 gene of S. cerevisiae was found to be essential for cell growth. The loss of YPT1 function, studied in cells with the YPT1 gene on chromosome VI regulated by the galactose-inducible GAL10 promoter, led to arrested cells that were multibudded and exhibited a complete disorganization of microtubules and an(More)
The YPT1 gene of the yeast Saccharomyces cerevisiae codes for a guanine nucleotide-binding protein which is essential for cell viability. Using as hybridization probe cloned yeast YPT1 gene sequences, we have isolated from cDNA libraries prepared from RNA of mouse F9 and C3H10T1/2 cells several overlapping cDNA clones with identical sequence in the regions(More)
Using site-directed mutagenesis, the ras-related and essential yeast YPT1 gene was changed to generate proteins with amino acid exchanges within conserved regions. Bacterially produced wild-type proteins were used for biochemical studies in vitro and were found to have properties very similar to mammalian ras proteins. Gene replacement allowed the study of(More)
In recent years there has been a substantial debate about the need for increasingly spontaneous, conversational corpora of spoken interaction that are not controlled or task directed. In parallel the need has arisen for the recording of multi-modal corpora which are not restricted to the audio domain alone. With a corpus that would fulfill both needs, it(More)
This paper describes a new open source architecture for unit-selection based speech synthesis called BOSS (Bonn Open Synthesis System). It is built up modularly, with communications between modules taking place in a fixed format. This makes the addition, deletion and substitution of modules very easy. The strict separation between data and algorithms allows(More)
In a previous study [1] we investigated properties of communicative feedback produced by attentive and non-attentive listeners in dialogue. Distracted listeners were found to produce less feedback communicating understanding. Here, we assess the role of prosody in differentiating between feedback functions. We find significant differences across all studied(More)