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Maize plants were grown in an autoclaved quartz sand–soil mix to which the bacterial communities of the soil and the mycorrhizal inocula were reintroduced. The root systems of the plants were divided with the two halves growing in separate pots. There were five different treatments: plants with both root halves non-mycorrhizal either at high or low P(More)
White lupin was grown in a quartz sand–soil mix with poorly available Ca phosphate. The plants were harvested on days 21, 35 and 51 and DNA was extracted from the non-cluster roots, the young, mature and senescent cluster roots with adhering soil. Bacterial community structure was examined by PCR-DGGE of 16S rDNA, digitisation of the band patterns and(More)
Mycorrhizal and non-mycorrhizal (NM) maize plants were grown for 4 or 7 weeks in an autoclaved quartz sand-soil mix. Half of the NM plants were supplied with soluble P (NM-HP) while the other half (NM-LP), like the mycorrhizal plants, received poorly soluble Fe and Al phosphate. The mycorrhizal plants were inoculated with Glomus mosseae or G. intraradices.(More)
This study investigates the small-scale stratification of bacterial community composition and functional diversity in the rhizosphere of maize. Maize seedlings were grown in a microcosm with a horizontal mesh (53 μM) creating a planar root mat and rhizosphere soil. An unplanted microcosm served as control. Thin slices of soil were cut at different distances(More)
Phytases are a group of enzymes capable of releasing phosphate from phytate, one of the most abundant forms of organic phosphate in the natural environment. Phytases can be found in many organisms; in bacteria, they are particularly described in g-proteobacteria. In recent years, bacterial phytases have been isolated, characterized and proposed as potential(More)
Plant genotypes differ in P efficiency, i.e. their capacity to grow in soil with low P availability. Plant properties such as root and root hair length, release of P mineralising and mobilising compounds by the roots and P requirement for optimal growth are known to influence P efficiency. In order to improve the understanding of the role of rhizosphere(More)
Two ectomycorrhizal fungi, Paxillus involutus 533 and Laccaria bicolor S238, differing greatly in their mycelial characteristics, were investigated with regard to their cation exchange capacity and Pb-binding capacity in vitro after growth with either NO3 - or NH4 + as N source. The CECs of 800–1200 μmol g-1 dry weight for Paxillus involutus 533 and(More)
Rhizosphere microbial communities are important for plant nutrition and plant health. Using the culture-independent method of PCR-DGGE of 16S rDNA for community analyses, we conducted several experiments to investigate the importance of pH, soil type, soil amendment, nutritional status of the plant, plant species and plant age on the structure of the(More)
The effects of agronomic management practices on the soil microbial community were investigated in a maize production system in New South Wales, Australia. The site has been intensively studied to measure the impact of stubble management and N-fertilizer application on greenhouse gas emissions (CO(2) and N(2)O), N-cycling, pathology, soil structure and(More)
Crop nutrition is frequently inadequate as a result of the expansion of cropping into marginal lands, elevated crop yields placing increasing demands on soil nutrient reserves, and environmental and economic concerns about applying fertilizers. Plants exposed to nutrient deficiency activate a range of mechanisms that result in increased nutrient(More)