Petra Bernasconi

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Proton pumping ATPases/ATPsynthases are found in all groups of present-day organisms. The structure of V- and F-type ATPases/ATP synthases is very conserved throughout evolution. Sequence analysis shows that the V- and F-type ATPases evolved from the same enzyme already present in the last common ancestor of all known extant life forms. The catalytic and(More)
The B subunit (approximately 60 kDa) of the vacuolar H(+)-ATPase is one of the two major subunits comprising the hydrophilic catalytic complex of the enzyme. Using left and catalytic complex of the enzyme. Using left and right primers which bind two highly conserved sequences of the B subunit, an 836-base pair fragment was amplified from human brain cDNA by(More)
Leaf-level microscopical symptom structure and physiological responses were investigated in seedlings experimentally exposed to ozone (O3) in indoor chambers (150 ppb, 8 h d(-1)/7 weeks), and field trees of Manna ash (Fraxinus ornus) exposed to ambient O3 (max 93 ppb/one growing season). Ozone-induced leaf injury, including leaf reddening and stippling, was(More)
We investigated the 5'-upstream region of the gene encoding the catalytic subunit of the V-type H(+)-ATPase in Daucus carota. A genomic sublibrary was screened with a cDNA probe, and a 4-kilobase genomic clone was obtained covering the first two exons and about 3 kilobases of the 5'-upstream sequence. The intron/exon boundaries matched established consensus(More)
We present stellar evolutionary models covering the mass range from 0.4 to 1 M ⊙ calculated for metal-licities Z=0.020 and 0.001 with the MHD equation of state (Hummer & Mihalas, 1988; Mihalas et al. 1988; Däppen et al. 1988). A parallel calculation using the OPAL (Rogers et al. 1996) equation of state has been made to demonstrate the adequacy of the MHD(More)
The formation of stars up to 120 M is computed in the framework of the accretion scenario. For realistic accretion rates derived from the observed line width in various molecular dark clouds, the accretion interlude lasts some 2-2.5 Myr, and accounts for an appreciable nuclear evolution during the optically thick MS life. Several new results are found(More)
Much previous research on recycling behavior has drawn heavily on models of personal and perceived social norms, as well as of personal attitudes, to explain recycling behavior. Although such models have received empirical support, the issue concerning discrepancies between norms, personal attitudes and an individual's behavior is yet to be resolved. Using(More)
An 800 bp fragment of Methanococcus thermolithotrophicus genomic DNA was amplified by the polymerase chain reaction method using primers designed from conserved regions of the V-type H+ ATPase regulatory subunits from the archaebacterium Sulfolobus, and several eukaryotes. Although more than one product was obtained, only one of them had the expected size(More)