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A piezoelectric sensor with immobilized polyclonal antibody was developed as a label-free assay for the model bacterium, Escherichia coli. The polyclonal antibody was prepared from mice BALB/c and covalently immobilized on the sensing gold electrode of the piezoelectric quartz crystal. The biosensor was able to detect E. coli in the range of 10(6)-10(9)(More)
There are three most important bacterial causative agents of serious infections that could be misused for warfare purposes: Bacillus anthracis (the causative agent of anthrax) is the most frequently mentioned one; however, Fracisella tularensis (causing tularemia) and Yersinia pestis (the causative agent of plague) are further bacterial agents enlisted by(More)
A novel immunosensing device based on a piezoelectric sensor for direct detection of the biological warfare agentFrancisella tularensis was developed. This sensor includes mouse polyclonal antibody immobilized in a layer of protein A covalently linked to the gold electrode of the sensor. The immunosensor is able to detectF. tularensis with the limit of(More)
Single chain Fv (scFv) antibodies (generated by phage display technology, molecules representing new and efficient tools in the research and diagnostics of infectious diseases) against the capsid protein (p25) of Maedi-Visna virus were selected. Several clones of p25 specific scFv antibodies were identified; one of them was expressed as a soluble scFv(More)
A single-chain antibody fragments (scFv) was developed directed against transmembrane envelope glycoprotein gp46 of the virus maedi-visna, by the application of the antibody phage display library. To get specific scFv binders, the library was panned against the biotinylated peptide of 20 amino acids corresponding to the principal immunodominant domain of(More)
A piezoelectric immunosensor for indirect diagnosis of tularemic infection in mouse serum was developed. Francisella tularensis LVS antigen was covalently immobilized on the sensing surface using cystamine and glutaraldehyde for activation and modification of the gold electrode. The normal mouse serum (NMS) and serum prepared from mice immunized by(More)
A highly sensitive piezoelectric biosensor has been developed for detection of cholinesterase inhibitors. The inhibitor benzoylecgonine-1,8-diamino-3,4-dioxaoctane (BZE-DADOO) was immobilized on a monolayer of 11-mercaptomonoundecanoic acid (MUA) self-assembled on the gold surface of the sensor. The binding of high-molecular-weight cholinesterase to the(More)
A new method of dissimilatory nitrite reductase (cytochrome cd1) isolation from the periplasmic fraction of anaerobically grown cells of the bacterium Paracoccus denitrificans was developed, using ionex and gel permeation chromatography with FPLC system (Pharmacia, Sweden). In experiments with isolated enzyme it was shown that through a nitrite reduction,(More)
Three sites of inhibitory action of hydroxylamine were identified in the respiratory chain of anaerobically grown bacterium Paracoccus denitrificans. Terminal oxidases were blocked at concentrations of 10(-4) to 10(-3) mol.l-1, and the inhibitor competed with artificial donor of electrons N, N, N', N'-tetramethyl-l, 4-phenylenediamine. In the anaerobic part(More)
The direct immunosensor for determination of the herbicide atrazine was studied. The gold electrodes of the piezoelectric quartz crystal were silanized and activated using glutaraldehyde. The bioaffinity ligand atrazine was linked through albumin as a spacer molecule. The modified piezoelectric crystal was placed in a flow cell and all measurements were(More)