Petr Rysavý

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Most nuclearly encoded mitochondrial proteins are synthesized with an amino-terminal leader peptide that is cleaved by the mitochondrial processing peptidase (MPP). Purified rat liver MPP, like the Neurospora and yeast enzymes, consists of two nonidentical subunits, alpha (55 kDa) and beta (50 kDa). To confirm the functional authenticity of the recently(More)
Rat liver mitochondrial processing peptidase (MPP) is the primary peptidase that cleaves leader peptides from nuclearly encoded mitochondrial proteins following their transport from the cytosol to the mitochondrial matrix. This enzyme consists of two nonidentical subunits that have overall similarity to each other and share certain amino acid motifs. These(More)
The microflora of the chernozem soil mineralized 62 % of the lactose retained on a column consisting of three 10-g layers of the soil at a daily flow of 48 mg of the sugar. Only 45 % of the sugar was mineralized when the daily flow was 136 mg. The highest value of the β-galactosidase activity in the system of heterocontinuous cultivation was two-fold with(More)
The activity of β-galactosidase during incubation of soil with lactose was studied. Enzyme activity reached its maximum between 6–24 h incubation. The addition of secondary ammonium phosphate stimulated the enzyme formation. Casein hydrolysate alone did not influence significantly enzyme formation even after a 72 h incubation. Enzyme activity in the(More)
The k-means algorithm is popular for data clustering applications. Most implementations use Lloyd’s algorithm, which does many unnecessary distance calculations. Several accelerated algorithms (Elkan’s, Hamerly’s, heap, etc.) have recently been developed which produce exactly the same answer as Lloyd’s, only faster. They avoid redundant work using the(More)
The ability of soil microflora to utilize glucose or cellobiose was found to depend on previous incubation of the soil with glucose, cellobiose or cellulose. Glucose was utilized more rapidly than cellobiose in soil preincubated with glucose or cellobiose. The opposite situation was observed in soil preincubated with cellulose. In the presence of a mixture(More)
We analyzed the folding, covalent flavinylation, and mitochondrial import of the rabbit reticulocyte lysate-translated bacterial 6-hydroxy-D-nicotine oxidase (6-HDNO) fused to the mitochondrial targeting sequence of rat liver dimethylglycine dehydrogenase. Translation of 6-HDNO in FAD-supplemented reticulocyte lysate resulted in a protein that contained(More)
Clustering biological sequences is a central task in bioinformatics. The typical result of new-generation sequencers is a set of short substrings (“reads”) of a target sequence, rather than the sequence itself. To cluster sequences given only their read-set representations, one may try to reconstruct each one from the corresponding read set, and then employ(More)
The microflora of the chernozem soil mineralized 62% of the lactose retained on a column consisting of three 10-g layers of the soil at a daily flow of 48 mg of the sugar. Only 45% of the sugar was mineralized when the daily flow was 136 mg. The highest value of the beta-galactosidase activity in the system of heterocontinuous cultivation was two-fold with(More)
The method for the assay of β-galactosidase introduced by Lederberg (1950) was modified and used for studies of the enzyme in the soil.o-Nitrophenyl-β-d-galactoside served as substrate. Trismaleinate buffer was found to be more suitable than phosphate buffer in the assay. The enzyme reaction was stopped by adding sulphuric acid and the incubation mixture(More)