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INTRODUCTION Hemojuvelin (Hjv) is a key component of the signaling cascade that regulates liver hepcidin (Hamp) expression. The purpose of this study was to determine Hjv protein levels in mice and rats subjected to iron overload and iron deficiency. METHODS C57BL/6 mice were injected with iron (200 mg/kg); iron deficiency was induced by feeding of an(More)
BACKGROUND Anderson-Fabry disease (AFD) is an X-linked genetic disorder with deficient α-galactosidase A activity. The main aim of this work was to investigate possible differences in urine proteins between healthy controls and AFD patients and to identify abnormal proteins as potential biomarkers of disease. MATERIAL AND METHODS We studied 2D(More)
Preparation of affinity polyacrylamide gels containing immobilized Fe(III) ions for the separation of proteins exhibiting metal ion binding properties is described. The presented method enables uniform distribution of immobilized metal ions in the affinity part of the polyacrylamide separating gel. Affinity gels prepared by this way are suitable to follow(More)
A long series of polar patches was observed by ionosondes and an all-sky imager during a disturbed period (Kp ˆ 7and IMF Bz < 0). The ionosondes measured electron densities of up to 9 ́ 10 m in the patch center, an increase above the density minimum between patches by a factor of 4.5. Bands of F-region irregularities generated at the equatorward edge of(More)
Magnetic bead cellulose was prepared by a suspension method from the mixture of viscose and magnetite using thermal sol-gel transition and regeneration of cellulose. The prepared magnetic particles after their activation with divinyl sulfone were shown to be suitable magnetic carrier for immobilization of α-chymotrypsin and for its application in proteomic(More)
Pulsed ionospheric flows (PIFs) in the cusp footprint have been observed by the SuperDARN radars with periods between a few minutes and several tens of minutes. PIFs are believed to be a consequence of the interplanetary magnetic field (IMF) reconnection with the magnetospheric magnetic field on the dayside magnetopause, ionospheric signatures of flux(More)
Normal human B lymphocytes are sensitive to the growth-inhibitory action of TGF-beta1 whereas malignant B lymphoma cells are mostly resistant to TGF-beta1 effects. We have shown in our previous work that, TGF-beta1 treatment resulted in significant growth inhibition of the DoHH2 cell line. In the present study we showed that TGF-beta1-induced growth arrest(More)
A case study of medium-scale travelling ionospheric disturbances (TIDs) that are correlated with solar wind Alfvén waves is presented. The HF radar groundscatter signatures of TIDs caused by atmospheric gravity waves with periods of 20–40 min are traced to a source at high latitudes, namely pulsed ionospheric flows (PIFs) due to bursts in the convection(More)
Matriptase-2 (TMPRSS6) is an important negative regulator of hepcidin expression; however, the effects of iron overload or accelerated erythropoiesis on liver TMPRSS6 protein content in vivo are largely unknown. We determined TMPRSS6 protein content in plasma membrane-enriched fractions of liver homogenates by immunoblotting, using a commercial antibody(More)
Iron overload causes tissue damage in the liver, but its initial effects at the molecular and cellular level are not well understood. Epithelial cadherin (E-cad) is a major adhesion protein in adherens junctions and is associated with several signal transduction pathways. Dysfunction of E-cad causes instability of adherens junctions, which leads to cell(More)