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The photochemistry of a major commercial polybrominated diphenyl ether (PBDE) flame retardant congener, 2,2',4,4',5,5'-hexabromodiphenyl ether (BDE153), was investigated in acetonitrile, distilled water, and seawater. After a short irradiation period in acetonitrile at 302 nm, the major photoproducts of BDE153 included 2,2',4,4',5-(BDE99),(More)
1-Acyl-7-nitroindolines have been found to be useful photoactivated protecting groups for rapid release of carboxylates in aqueous solution. Mechanistic details of carboxylic acid photorelease from model compounds in solutions of varying H2O-CH3CN composition are now reported, using data from product studies, deuterium isotope effects, kinetic studies(More)
Both NADH- and NADPH-dependent Baeyer-Villiger monooxygenase activities with potential uses as biocatalysts for biotransformations are present to different extents throughout the growth of Pseudomonas putida NCIMB 10007 on (+)-camphor. The two activities give a different pattern of stereoselective oxygenations with various mono- and bicyclic ketone(More)
BACKGROUND Gossypol, a natural compound present in cottonseeds, displays antiproliferative and pro-apoptotic effects against various cancer cells. The (-)-gossypol enantiomer is a more potent inhibitor of cancer cell growth. Here, the molecular mechanisms of apoptosis induced by (-)-gossypol were studied in human prostate cancer cells. MATERIALS AND(More)
Ultracentrifugation, membrane osmometry and capillary viscometry experiments have been performed on two dextran samples, which have molecular-weight distributions (MWDs) similar to those of dextrans used as blood plasma extenders. The manufacturer reported values of Mn and MW, determined by end group analysis and by light scattering, respectively. Our(More)
Natural gossypol (GP), a polyphenolic pigment in cottonseed, is a racemic mixture of two enantiomers, (+)GP and (-)GP. Our aim was to compare the abilities of (+/-)GP, (+)GP and (-)GP to reduce proliferation of breast cancerous epithelial cells (cEC) and cancerous stromal cells (cSC). Proliferation was measured by 3H-thymidine uptake. Results showed that(More)
BACKGROUND Acquisition of metastatic ability by prostatic cancer cells is the most lethal aspect of prostatic cancer progression. (-)-Gossypol, a polyphenolic compound present in cottonseeds, possesses anti-proliferative and proapoptotic effects in various cancer cells. MATERIALS AND METHODS In this study, the differences between MAT-LyLu, rat prostate(More)
BACKGROUND Gossypol, a natural polyphenolic compound present in cottonseeds, possesses antiproliferative and pro-apoptotic effects in in vivo and in vitro models. There are two enantiomers, (+)-gossypol and (-)-gossypol, the latter being a more potent inhibitor of cancer cell growth. Here, the effect of bovine serum albumin (BSA) and dextran-coated(More)
Anti-gossypol monoclonal antibody was purified from cell culturing supernatant by ammonium sulfate precipitation and Protein A AffinityPak. The antigen (i.e., gossypol) was labeled with horseradish peroxidase through Schiff-base formation. Both the purified antibody and the enzyme-labeled gossypol were employed to develop a competitive direct enzyme-linked(More)