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FK506 (tacrolimus) is a secondary metabolite with a potent immunosuppressive activity, currently registered for use as immunosuppressant after organ transplantation. FK506 and FK520 are biogenetically related natural products that are synthesized by combined polyketide synthase/nonribosomal peptide synthetase systems. The entire gene cluster for(More)
FK506 (Tacrolimus) is an important immunosuppressant, produced by industrial biosynthetic processes using various Streptomyces species. Considering the complex structure of FK506, it is reasonable to expect complex regulatory networks controlling its biosynthesis. Regulatory elements, present in gene clusters can have a profound influence on the final yield(More)
Oscillation-based testing of analog-to-digital converters represents a viable option for low-cost built-in self-testing in mixed-signal design. While numerous papers have addressed implementation issues, little attention has been paid to the measurement accuracy. In this letter, we highlight an inherent measurement uncertainty which has to be considered(More)
Employing DERA (2-deoxyribose-5-phosphate aldolase), we developed the first whole-cell biotransformation process for production of chiral lactol intermediates useful for synthesis of optically pure super-statins such as rosuvastatin and pitavastatin. Herein, we report the development of a fed-batch, high-density fermentation with Escherichia coli BL21 (DE3)(More)
Testing of a deeply embedded mixed-signal core in a System-on-Chip (SoC) is a challenging issue due to the communication bottleneck in accessing the core from external automatic test equipment. Consequently, in many cases the preferred approach is built-in self-test (BIST), where the major part of test activity is performed within the unit-under-test and(More)
Temperature Simulations in Cooling Appliances The R&D laboratory of Gorenje d. d. develops complex cooling appliances. In their developing process there are also engineers from other areas participating. Their task is to develop optimal-performance appliances, regarding all func-tionalities. To allow for an easier and faster development, we used(More)
In this paper we present a program tool that was developed for the simulation of temperatures inside the cooling appliance. The simulator is used to check the performance of the appliance at different settings of control parameters. These simulations replace the part of the measurements, which are time consuming due to the slow thermal processes, but are(More)
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