Peter J Fisher

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 Dinucleotide microsatellites were isolated from Pinus radiata using both a standard genomic library and libraries enriched for microsatellites. Locus-specific primers were designed to amplify 43 unique microsatellites. Thirty two of these loci had interpretable PCR patterns, 11 of which were polymorphic in a screen of 19 P. radiata individuals; all 11(More)
Endophytic fungi were isolated from healthy leaves of Manilkara bidentata (Sapotaceae) collected in Puerto Rico. One leaf was collected from each of three trees. Four 5 X 20 mm panels were cut from each leaf, surface sterilized, cut into 50 1 X 2 mm pieces, and plated on malt agar. Petioles were cut into ten 1 mm segments. Fungi were isolated from 90%-95%(More)
The relationship between metabolite production and genotypic diversity in two endophytic fungi was investigated. We selected populations of Cylindrocarpon destructans and Heliscus lugdunensis from the roots of a single tree. A total of 49 isolates of both species were selected and classified by simple genotypic tests (random amplified polymorphic DNA(More)
Calmodulin binds to amphiphilic, helical peptides of a variety of amino-acid sequences. These peptides are usually positively charged, although there is spectroscopic evidence that at least one neutral peptide binds. The complex between calmodulin and one of its natural target peptides, the binding site for calmodulin on smooth muscle myosin light-chain(More)
The formation of graphene on the (0001) surface of SiC (the Si-face) is studied by atomic force microscopy, low-energy electron microscopy, and scanning tunneling microscopy/spectroscopy. The graphene forms due to preferential sublimation of Si from the surface at high temperature, and the formation has been studied in both high-vacuum and 1-atm-argon(More)
Mixed micelles of the 26-residue, lytic peptide melittin (MLT) and 1-myristoyl-2-hydroxyl-sn-glycero-3-phosphocholine (MMPC) in aqueous solution at 25 degrees C were investigated by (13)C- and (31)P-NMR spectroscopy. (13)C alpha chemical shifts of isotopically labeled synthetic MLT revealed that MLT in the micelle is predominantly alpha-helical and that the(More)
Exposure of the aquatic hyphomycete Heliscus lugdunensis to the herbicide Mecoprop did not significantly affect production of the antigen recognized by the specific monoclonal antibody NG-CF10. Therefore, an ELISA method, developed in a previous study, could be used to quantify the biomass of H. lugdunensis colonizing leaves exposed to this herbicide.(More)
We fabricated high-mobility field-effect transistors based on epitaxial graphene synthesized by vacuum graphitization of both the Siand C-faces of SiC. Roomtemperature field-effect mobilities > 4,000 cm/Vs for both electrons and holes were achieved, although with wide distributions. By using a high-k gate dielectric, we were able to measure transistor(More)
A genus-specific monoclonal antibody, NG-CF10, raised in a previous study to the fungal pathogen Nectria galligena, was found to recognize the aquatic hyphomycete Heliscus lugdunensis (anamorph) and its teleomorph Nectria lugdunensis. Using this MAb in a plate trapped antigen- ELISA we could detect and determine the biomass of Heliscus lugdunensis in mixed(More)
Anionic polysaccharides, traditionally obtained from plant or algal sources, have a variety of commercial uses. Such gums from microorganisms have received increased recent interest. We have initiated a program to investigate the bioconversion of pentosans to rheologically useful anionic extracellular polysaccharides (AEPS). A number of earlier-described(More)