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Contrary to a previous report, electron microscopic studies on the Fny strain of cucumber mosaic virus (CMV)-infected tobacco tissues revealed that plasmodesmata were not structurally modified during CMV infection, nor were virions ever observed in plasmodesmata connecting infected cells. To further explore the basis of CMV infection, experiments were(More)
Plant viruses often contain parasites of their own, referred to as satellites. Satellite RNAs are dependent on their associated (helper) virus for both replication and encapsidation. Satellite RNAs vary from 194 to approximately 1,500 nucleotides (nt). The larger satellites (900 to 1,500 nt) contain open reading frames and express proteins in vitro and in(More)
The organization of the intergenic spacer of a 9.04 kb tomato ribosomal RNA gene (rDNA) was determined. The 3258 by spacer contains two major repeat elements enclosing a region which includes 351 by of an 81.8% A–T rich sequence. A block of nine 53 by repeats begins 388 by downstream from the 3′ end of the 25S rRNA. The A–T rich domain is followed by a(More)
Some strains of cucumber mosaic virus (CMV) induce a bright yellow/white chlorosis in tobacco instead of the light green/dark green mosaic induced by most CMV strains. This property is controlled by RNA 3 of this tripartite virus. Recombination between cDNA clones of RNA 3 from a green mosaic strain, Fny-CMV, and a chlorotic strain, M-CMV, and inoculation(More)
Cucumber mosaic cucumovirus (CMV) can be divided into two subgroups, I and II. LS-CMV and most other subgroup II strains cause a mild, systemic mottle on tobacco and can induce a necrotic etching (necrotic rings) symptom on inoculated tobacco leaves. In contrast, Fny-CMV and most other subgroup I strains cause severe, systemic mosaic symptoms on tobacco,(More)
A mutant of the Cucumber mosaic virus subgroup IA strain Fny (Fny-CMV) lacking the gene encoding the 2b protein (Fny-CMVdelta2b) induced a symptomless systemic infection in tobacco. Both the accumulation of Fny-CMVdelta2b in inoculated tissue and the systemic movement of the virus appeared to proceed more slowly than for wild-type Fny-CMV. The influence of(More)
Many scientists, if not all, feel that their particular plant virus should appear in any list of the most important plant viruses. However, to our knowledge, no such list exists. The aim of this review was to survey all plant virologists with an association with Molecular Plant Pathology and ask them to nominate which plant viruses they would place in a(More)
The cucumber mosaic virus (CMV) 3a movement protein (MP) was compared directly to the well-characterized tobacco mosaic virus (TMV) 30K MP by cloning the genes encoding these proteins into Escherichia coli, isolating the E. coli-expressed MPs, and characterizing them with regard to RNA- and NTP-binding activities. The two MPs were shown to bind(More)