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The Complete Spectrum of Yeast Chromosome Instability Genes Identifies Candidate CIN Cancer Genes and Functional Roles for ASTRA Complex Components
The comprehensive CIN gene list presented here in principle comprises all conserved eukaryotic genome integrity pathways and allows direct cross-referencing with tumor mutational data and thus candidate mutations potentially driving CIN in tumors.
Genome-Wide Profiling of Yeast DNA:RNA Hybrid Prone Sites with DRIP-Chip
The genome-wide distribution of DNA:RNA hybrid prone loci in Saccharomyces cerevisiae is described by DNA: RNA immunoprecipitation (DRIP) followed by hybridization on tiling microarray, showing that DNA:RNAs hybrids preferentially accumulated at rDNA, Ty1 and Ty2 transposons, telomeric repeat regions and a subset of open reading frames (ORFs).
R-loop-mediated genome instability in mRNA cleavage and polyadenylation mutants.
It is found that truncation fusions of yeast FIP1 analogous to those in cancer cause loss of function and that siRNA knockdown of FIP 1L1 in human cells increases DNA damage and chromosome breakage.
CX-5461 is a DNA G-quadruplex stabilizer with selective lethality in BRCA1/2 deficient tumours
CX-5461 is a G-quadruplex stabilizer, with specific toxicity against BRCA deficiencies in cancer cells and polyclonal patient-derived xenograft models, including tumours resistant to PARP inhibition.
The interaction network of the chaperonin CCT
This study uses proteomic and genomic approaches to define CCT interaction networks involving 136 proteins/genes that include links to the nuclear pore complex, chromatin remodelling, and protein degradation that provide a rich framework for understanding the function of CCT in several essential cellular processes, including epigenetics and cell division.
PhLP3 Modulates CCT-mediated Actin and Tubulin Folding via Ternary Complexes with Substrates*
It is proposed that the antagonistic actions of PhLP3 and prefoldin serve to modulate CCT activity and play a key role in establishing a functional cytoskeleton in vivo.
Molecular clamp mechanism of substrate binding by hydrophobic coiled-coil residues of the archaeal chaperone prefoldin.
It is shown that the six coiled-coil tentacles of archaeal PFD act in concert to bind and stabilize nonnative proteins near the opening of the cavity they form.