Petar Tomev Mitrikeski

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The Balkan Peninsula is one of three major European refugial areas. It has high biodiversity and endemism, but data on the age and origin of its fauna, especially endemics, are limited. Mitochondrial sequence data (COI and 16S genes) were used to study the population structure and phylogeography of the caddisfly Drusus croaticus and the phylogeny and(More)
The demonstration of spontaneous yeast competence shows that artificial transformation rests on naturally occurring cellular processes. Such natural competence is either biologically mediated or environmentally induced. For instance, wild yeast might be transformed through conjugation by cell-to-cell contact mediated either by Escherichia coli or(More)
We studied the influence of homology size and polymorphism on the integration of circular plasmids into the yeast CYC1 region. The plasmids used also contained the URA3 gene, and the proportion of Ura+ transformants resulting from plasmid integration into the CYC1 region was determined by Southern-blot analysis. A size-dependent decrease in integration into(More)
Recombination of non-replicative plasmids bearing yeast homology with the chromosome can integrate the plasmid molecule into the genome. Such process is also known to integrate more than one plasmid molecule leading to multiple, tandem plasmid integration. However, its exact molecular mechanism remains unknown. There are two alternative models to explain(More)
Unlike prokaryotes, eukaryotic organisms do not seem to be equipped with natural cell process(es) designated for exogenous DNA uptake. However, it is barely known that under laboratory circumstances resembling wild fungal environment(s), at least some lower eukaryotes could become naturally competent for exogenous DNA uptake. Thus, apart from the known fact(More)
Sporulation efficiency in the yeast Saccharomyces cerevisiae is a well-established model for studying quantitative traits. A variety of genes and nucleotides causing different sporulation efficiencies in laboratory, as well as in wild strains, has already been extensively characterised (mainly by reciprocal hemizygosity analysis and nucleotide exchange(More)
In bacteria, the RecA protein forms recombinogenic filaments required for the SOS response and DNA recombination. In order to form a recombinogenic filament, wild type RecA needs to bind ATP and to interact with mediator proteins. The RecA730 protein is a mutant version of RecA with superior catalytic abilities, allowing filament formation without the help(More)
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