Peng Shi

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The ability to pattern multiple bioactive cues on a surface is valuable for understanding how neurons interact with their complex extracellular environment. In this report, we introduce a set of methods for creating such surfaces, with the goals of understanding how developing neurons integrate multiple biologically relevant signals and as a tool for(More)
Assembly of functional neural circuits relies on the ability of axons to navigate a complex landscape of guidance cues in the extracellular environment. In this report, we investigate localized cell signaling in response to these cues by combining a microfabricated compartmentalization chamber with multicomponent, protein-micropatterned surfaces; this(More)
Recreation of neural network in vitro with designed topology is a valuable tool to decipher how neurons behave when interacting in hierarchical networks. In this study, we developed a simple and effective platform to pattern primary neurons in array formats for interrogation of neural circuitry with single cell resolution. Unlike many(More)
Formation of functional motor circuits relies on the ability of distinct spinal motor neuron subtypes to project their axons with high precision to appropriate muscle targets. While guidance cues contributing to motor axon pathfinding have been identified, the intracellular pathways underlying subtype-specific responses to these cues remain poorly(More)
Improving drought tolerance through drought preconditioning is an important way to understand drought tolerance mechanism in plants. Some limited researches have focused on the gene expression patterns in conjunction with the underlying enzymes promoting drought tolerance and post-drought recovery in white clover. The objective of this study was to identify(More)
In cognitive networks, cooperative spectrum sensing can increase detection performance significantly. In this paper, square-law combining technique is chosen as cooperative diversity scheme in energy detecting when the wireless environment is modeled as Nakagami channel. Moreover, we use double-threshold algorithm to reduce the effect caused by noise(More)
Control of cell migration is important in numerous key biological processes, and is implicated in pathological conditions such as cancer metastasis and inflammatory diseases. Many previous studies indicated that cell migration could be guided by micropatterns fabricated on cell culture surfaces. In this study, we designed a polydimethylsiloxane cell culture(More)
The reconstruction of neural activity across complete neural circuits, or brain activity mapping, has great potential in both fundamental and translational neuroscience research. Larval zebrafish, a vertebrate model, has recently been demonstrated to be amenable to whole brain activity mapping in behaving animals. Here we demonstrate a microfluidic array(More)
STUDY DESIGN Histological features of the intervertebral disc (IVD)-endplate interface were analyzed. OBJECTIVE To define cartilaginous and bony vertebral endplate in commonly used laboratory animals and compare with that of the humans. SUMMARY OF BACKGROUND DATA Endplates are crucial for the IVD nutrient supply: the IVDs have limited blood supply; most(More)
A combinatorial approach was used to present primary neurons with a large library of topographical features in the form of micropatterned substrate for high-throughput screening of physical neural-guidance cues that can effectively promote different aspects of neuronal development, including axon and dendritic outgrowth. Notably, the neuronal-guidance(More)